NDLI: Quantitative Analysis of Viral Load per Haploid Genome Revealed the Different Biological Features of Merkel Cell Polyomavirus Infection in Skin Tumor

Content Provider	PubMed Central
Author	Ota, Satoshi Ishikawa, Shumpei Takazawa, Yutaka Goto, Akiteru Fujii, Takeshi Ohashi, Ken-ichi Fukayama, Masashi
Editor	Toland, Amanda Ewart
Copyright Year	2012
Abstract	Merkel cell polyomavirus (MCPyV) has recently been identified in Merkel cell carcinoma (MCC), an aggressive cancer that occurs in sun-exposed skin. Conventional technologies, such as polymerase chain reaction (PCR) and immunohistochemistry, have produced conflicting results for MCPyV infections in non-MCC tumors. Therefore, we performed quantitative analyses of the MCPyV copy number in various skin tumor tissues, including MCC (n = 9) and other sun exposure-related skin tumors (basal cell carcinoma [BCC, n = 45], actinic keratosis [AK, n = 52], Bowen’s disease [n = 34], seborrheic keratosis [n = 5], primary cutaneous anaplastic large-cell lymphoma [n = 5], malignant melanoma [n = 5], and melanocytic nevus [n = 6]). In a conventional PCR analysis, MCPyV DNA was detected in MCC (9 cases; 100%), BCC (1 case; 2%), and AK (3 cases; 6%). We then used digital PCR technology to estimate the absolute viral copy number per haploid human genome in these tissues. The viral copy number per haploid genome was estimated to be around 1 in most MCC tissues, and there were marked differences between the MCC (0.119–42.8) and AK (0.02–0.07) groups. PCR-positive BCC tissue showed a similar viral load as MCC tissue (0.662). Immunohistochemistry with a monoclonal antibody against the MCPyV T antigen (CM2B4) demonstrated positive nuclear localization in most of the high-viral-load tumor groups (8 of 9 MCC and 1 BCC), but not in the low-viral-load or PCR-negative tumor groups. These results demonstrated that MCPyV infection is possibly involved in a minority of sun-exposed skin tumors, including BCC and AK, and that these tumors display different modes of infection.
Related Links	http://dx.doi.org/10.1371/journal.pone.0039954
Starting Page	39954
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	6
Volume Number	7
Language	English
Publisher	Public Library of Science
Publisher Date	2012-06-29
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A cornucopia of human polyomaviruses.

Merkel cell polyomavirus in cutaneous swabs.

The Merkel Cell Polyomavirus T Antigens Function as Tumor Promoters in Murine Skin

Merkel cell polyomavirus-positive Merkel cell carcinoma requires viral small T-antigen for cell proliferation.

Immunohistochemical features of Merkel cell carcinoma in correlation with presence of Merkel cell polyomavirus DNA.

Cancer. A skin cancer virus?

Positive correlation between Merkel cell polyomavirus viral load and capsid-specific antibody titer

Clonal integration of a polyomavirus in human Merkel cell carcinoma.

Merkel cell polyomavirus strains in patients with merkel cell carcinoma.

Quantitative Analysis of Viral Load per Haploid Genome Revealed the Different Biological Features of Merkel Cell Polyomavirus Infection in Skin Tumor

Similar Documents

A cornucopia of human polyomaviruses.

Merkel cell polyomavirus in cutaneous swabs.

The Merkel Cell Polyomavirus T Antigens Function as Tumor Promoters in Murine Skin

Merkel cell polyomavirus-positive Merkel cell carcinoma requires viral small T-antigen for cell proliferation.

Immunohistochemical features of Merkel cell carcinoma in correlation with presence of Merkel cell polyomavirus DNA.

Cancer. A skin cancer virus?

Positive correlation between Merkel cell polyomavirus viral load and capsid-specific antibody titer

Clonal integration of a polyomavirus in human Merkel cell carcinoma.

Merkel cell polyomavirus strains in patients with merkel cell carcinoma.

Quantitative Analysis of Viral Load per Haploid Genome Revealed the Different Biological Features of Merkel Cell Polyomavirus Infection in Skin Tumor