NDLI: Room temperature femtosecond X-ray diffraction of photosystem II microcrystals

Content Provider	PubMed Central
Author	Kern, Jan Roberto, Alonso-mori Hellmich, Julia Tran, Rosalie Johan, Hattne Hartawan, Laksmono Carina, Glöckner Echols, Nathaniel Sierra, Raymond G. Sellberg, Jonas Benedikt, Lassalle-kaiser Gildea, Richard J. Glatzel, Pieter Grosse-kunstleve, Ralf W. Latimer, Matthew J. Mcqueen, Trevor A. Difiore, Dörte Fry, Alan R. Messerschmidt, Marc Alan, Miahnahri Schafer, Donald W. Seibert, M. Marvin Sokaras, Dimosthenis Weng, Tsu-chien Zwart, Petrus H. White, William E. Adams, Paul D. Bogan, Michael J. Boutet, Sébastien Williams, Garth J. Messinger, Johannes Sauter, Nicholas K. Zouni, Athina Bergmann, Uwe Yano, Junko Yachandra, Vittal K.
Abstract	Most of the dioxygen on earth is generated by the oxidation of water by photosystem II (PS II) using light from the sun. This light-driven, four-photon reaction is catalyzed by the Mn4CaO5 cluster located at the lumenal side of PS II. Various X-ray studies have been carried out at cryogenic temperatures to understand the intermediate steps involved in the water oxidation mechanism. However, the necessity for collecting data at room temperature, especially for studying the transient steps during the O–O bond formation, requires the development of new methodologies. In this paper we report room temperature X-ray diffraction data of PS II microcrystals obtained using ultrashort (< 50 fs) 9 keV X-ray pulses from a hard X-ray free electron laser, namely the Linac Coherent Light Source. The results presented here demonstrate that the ”probe before destroy” approach using an X-ray free electron laser works even for the highly-sensitive Mn4CaO5 cluster in PS II at room temperature. We show that these data are comparable to those obtained in synchrotron radiation studies as seen by the similarities in the overall structure of the helices, the protein subunits and the location of the various cofactors. This work is, therefore, an important step toward future studies for resolving the structure of the Mn4CaO5 cluster without any damage at room temperature, and of the reaction intermediates of PS II during O–O bond formation.
Related Links	http://dx.doi.org/10.1073/pnas.1204598109
Starting Page	9721
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	25
Volume Number	109
Language	English
Publisher	National Academy of Sciences
Publisher Date	2012-06-19
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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