NDLI: Spheres Derived from Lung Adenocarcinoma Pleural Effusions: Molecular Characterization and Tumor Engraftment

Content Provider	PubMed Central
Author	Mancini, Rita Enrico, Giarnieri De Vitis, Claudia Malanga, Donatella Giuseppe, Roscilli Noto, Alessia Marra, Emanuele Carmelo, Laudanna Pietro, Zoppoli Luca, Pasquale De Affuso, Andrea Luigi, Ruco Napoli, Arianna Di Mesiti, Giuseppe Luigi, Aurisicchio Ricci, Alberto Salvatore, Mariotta Pisani, Lara Claudio, Andreetti Giuseppe, Viglietto Rendina, Erino A. Giovagnoli, Maria Rosaria Ciliberto, Gennaro
Editor	Tang, Dean G.
Copyright Year	2011
Abstract	Malignant pleural effusions (MPEs) could represent an excellent source to culture a wide variety of cancer cells from different donors. In this study, we set up culture conditions for cancer cells deriving from MPEs of several patients affected by the most frequent form of lung cancer, namely the subset of non small cell lung cancers (NSCLC) classified as Lung Adenocarcinomas (AdenoCa) which account for approximately 40% of lung cancer cases. AdenoCa malignant pleural effusions gave rise to in vitro cultures both in adherent and/or in spheroid conditions in almost all cases analyzed. We characterized in greater detail two samples which showed the most efficient propagation in vitro. In these samples we also compared gene profiles of spheroid vs adherent cultures and identified a set of differentially expressed genes. Finally we achieved efficient tumor engraftment in recipient NOD/SCID mice, also upon inoculation of small number of cells, thus suggesting indirectly the presence of tumor initiating cells.
Related Links	http://dx.doi.org/10.1371/journal.pone.0021320
Starting Page	21320
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	7
Volume Number	6
Language	English
Publisher	Public Library of Science
Publisher Date	2011-07-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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