NDLI: Spatiotemporal measurement of freezing-induced deformation of engineered tissues

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Spatiotemporal measurement of freezing-induced deformation of engineered tissues

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Spatiotemporal measurement of freezing-induced deformation of engineered tissues

Content Provider	PubMed Central
Author	Teo, Ka Yaw Dutton, J. Craig Han, Bumsoo
Abstract	In order to cryopreserve functional engineered tissues (ETs), the microstructure of the extracellular matrix (ECM) should be maintained as well as the cellular viability since the functionality is closely related to the ECM microstructure. Since the post-thaw ECM microstructure is determined by the deformation of ETs during cryopreservation, freezing-induced deformation of ETs was measured with a newly developed quantum dot (QD)-mediated cell image deformetry system using dermal equivalents as a model tissue. The dermal equivalents were constructed by seeding QD-labeled fibroblasts in type I collagen matrices. After 24 hour incubation, the ETs were directionally frozen by exposing them to a spatial temperature gradient (from 4 °C to −20 °C over a distance of 6 mm). While being frozen, the ETs were consecutively imaged, and consecutive pairs of these images were two-dimensionally cross-correlated to determine the local deformation during freezing. The results showed that freezing induced the deformation of ET, and its magnitude varied with both time and location. The maximum local dilatation was 0.006 s−1 and was always observed at the phase change interface. Due to this local expansion, the unfrozen region in front of the freezing interface experienced compression. This expansion-compression pattern was observed throughout the freezing process. In the unfrozen region, the deformation rate gradually decreased away from the freezing interface. After freezing/thawing, the ET experienced an approximately 28% decrease in thickness and 8% loss in weight. These results indicate that freezing-induced deformation caused the transport of interstitial fluid and the interstitial fluid was extruded. In summary, the results suggest that complex cell-fluid-matrix interactions occur within ETs during freezing, and these interactions determine the post-thaw ECM microstructure and eventual post-thaw tissue functionality.
Related Links	http://dx.doi.org/10.1115/1.4000875
Starting Page	31003
File Format	PDF
ISSN	01480731
e-ISSN	15288951
Journal	Journal of biomechanical engineering
Issue Number	3
Volume Number	132
Language	English
Publisher Date	2010-01-01
Access Restriction	Open
Subject Keyword	Physiology (medical) Biomedical Engineering Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology (medical) Biomedical Engineering

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