NDLI: Role of Cells in Freezing-Induced Cell-Fluid-Matrix Interactions Within Engineered Tissues

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Ozcelikkale, Altug Dutton, Craig Han, Bumsoo
Copyright Year	2013
Abstract	During cryopreservation, ice forms in the extracellular space resulting in freezing-induced deformation of the tissue, which can be detrimental to the extracellular matrix (ECM) microstructure. Meanwhile, cells dehydrate through an osmotically driven process as the intracellular water is transported to the extracellular space, increasing the volume of fluid for freezing. Therefore, this study examines the effects of cellular presence on tissue deformation and investigates the significance of intracellular water transport and cell-ECM interactions in freezing-induced cell-fluid-matrix interactions. Freezing-induced deformation characteristics were examined through cell image deformetry (CID) measurements of collagenous engineered tissues embedded with different concentrations of MCF7 breast cancer cells versus microspheres as their osmotically inactive counterparts. Additionally, the development of a biophysical model relates the freezing-induced expansion of the tissue due to the cellular water transport and the extracellular freezing thermodynamics for further verification. The magnitude of the freezing-induced dilatation was found to be not affected by the cellular water transport for the cell concentrations considered; however, the deformation patterns for different cell concentrations were different suggesting that cell-matrix interactions may have an effect. It was, therefore, determined that intracellular water transport during freezing was insignificant at the current experimental cell concentrations; however, it may be significant at concentrations similar to native tissue. Finally, the cell-matrix interactions provided mechanical support on the ECM to minimize the expansion regions in the tissues during freezing.
Starting Page	091001
Ending Page	091012
Page Count	12
File Format	PDF
ISSN	01480731
e-ISSN	15288951
Journal	Journal of Biomechanical Engineering
Volume Number	135
Issue Number	9
DOI	10.1115/1.4024571
Language	English
Publisher	The American Society of Mechanical Engineers
Publisher Date	2013-07-10
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biological tissues Cryonics Deformation Fluids Freezing Ice Temperature Water
Content Type	Text
Resource Type	Article
Subject	Physiology (medical) Biomedical Engineering

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