NDLI: Hepatitis B virus (HBV)-specific cytotoxic T-cell response in humans: production of target cells by stable expression of HBV-encoded proteins in immortalized human B-cell lines.

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Sickness and recovery of dogs challenged with a street rabies virus after vaccination with a vaccinia virus recombinant expressing rabies virus N protein.

Heterologous recombination in the double-stranded RNA bacteriophage phi 6.

In vitro packaging and replication of individual genomic segments of bacteriophage phi 6 RNA.

Molecular attenuation of vaccinia virus: mutant generation and animal characterization.

Expression of the Marek's disease virus (MDV) homolog of glycoprotein B of herpes simplex virus by a recombinant baculovirus and its identification as the B antigen (gp100, gp60, gp49) of MDV.

ts1, a temperature-sensitive mutant of Moloney murine leukemia virus TB, can infect both CD4+ and CD8+ T cells but requires CD4+ T cells in order to cause paralysis and immunodeficiency.

Bovine immunodeficiency-like virus encodes factors which trans activate the long terminal repeat.

Identification and characterization of the herpes simplex virus type 1 virion protein encoded by the UL35 open reading frame.

Specific lysis of targets expressing varicella-zoster virus gpI or gpIV by CD4+ human T-cell clones.

Hepatitis B virus (HBV)-specific cytotoxic T-cell response in humans: production of target cells by stable expression of HBV-encoded proteins in immortalized human B-cell lines.

Ovine lentivirus is macrophagetropic and does not replicate productively in T lymphocytes.

Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts.

Mutations within the 5' half of the avian retrovirus MC29 v-myc gene alter or abolish transformation of chicken embryo fibroblasts and macrophages.

Association of ICP0 but not ICP27 with purified virions of herpes simplex virus type 1.

The vaccinia virus B1R gene product is a serine/threonine protein kinase.

Nucleotide 880 splice donor site required for efficient transformation and RNA accumulation by human papillomavirus type 16 E7 gene.

Human immunodeficiency virus vectors for inducible expression of foreign genes.

Structural and functional characterization of the poliovirus replication complex.

Recombinant vaccine for canine parvovirus in dogs.

Stable rescue of a glycoprotein gII deletion mutant of pseudorabies virus by glycoprotein gI of bovine herpesvirus 1.

Immediate-early RNA 2.9 and early RNA 2.6 of bovine herpesvirus 1 are 3' coterminal and encode a putative zinc finger transactivator protein.

Establishment and functional characterization of human herpesvirus 6-specific CD4+ human T-cell clones.

Transformation of a continuous rat embryo fibroblast cell line requires three separate domains of simian virus 40 large T antigen.

Formation of heterotrimers between the membrane-integrated and the soluble glycoproteins of vesicular stomatitis virus leads to their intracellular cotransport.

Molecular characterization of a neuropathogenic and nonerythroleukemogenic variant of Friend murine leukemia virus PVC-211.

Molecular cloning and expression of a murine homolog of the human poliovirus receptor gene.

A second origin of DNA plus-strand synthesis is required for optimal human immunodeficiency virus replication.

Transformation-dependent expression of interleukin genes delivered by a recombinant parvovirus.

Duck hepatitis B virus infection of hepatocytes is not dependent on low pH.

Regulation of the Epstein-Barr virus DNA polymerase gene.

Identification of critical cis elements involved in mediating Epstein-Barr virus nuclear antigen 2-dependent activity of an enhancer located upstream of the viral BamHI C promoter.

Functional study of hepatitis delta virus large antigen in packaging and replication inhibition: role of the amino-terminal leucine zipper.

The insertion site of the temperate phage HB-746 is located near the phage remnant in the pneumococcal host chromosome.

Hemagglutinin-esterase-specific monoclonal antibodies alter the neuropathogenicity of mouse hepatitis virus.

Driving adenovirus type 12-transformed BALB/c mouse cells to express high levels of class I major histocompatibility complex proteins enhances, rather than abrogates, their tumorigenicity.

Insertional mutagenesis of flvi-2 in tumors induced by infection with LC-FeLV, a myc-containing strain of feline leukemia virus.

Use of second-site homologous recombination to demonstrate that Epstein-Barr virus nuclear protein 3B is not important for lymphocyte infection or growth transformation in vitro.

Herpes simplex virus type 1 ICP0 regulates expression of immediate-early, early, and late genes in productively infected cells.

Herpes simplex viruses with mutations in the gene encoding ICP0 are defective in gene expression.

Target epitope in the Tax protein of human T-cell leukemia virus type I recognized by class I major histocompatibility complex-restricted cytotoxic T cells.

Regulated expression of foreign genes in vaccinia virus under the control of bacteriophage T7 RNA polymerase and the Escherichia coli lac repressor.

Relationship of eukaryotic initiation factor 3 to poliovirus-induced p220 cleavage activity.

Cytotoxicity of a replication-defective mutant of herpes simplex virus type 1.

Viral and host cellular transcription in Autographa californica nuclear polyhedrosis virus-infected gypsy moth cell lines.

Effect of genomic location on expression of beta-galactosidase mRNA controlled by the herpes simplex virus type 1 UL38 promoter.

Ternary complex formation by vaccinia virus RNA polymerase at an early viral promoter: analysis by native gel electrophoresis.

Modification of some biological properties of HeLa cells containing adeno-associated virus DNA integrated into chromosome 17.

Characterization of a 7-kilodalton subunit of vaccinia virus DNA-dependent RNA polymerase with structural similarities to the smallest subunit of eukaryotic RNA polymerase II.

An early event in murine cytomegalovirus replication inhibits presentation of cellular antigens to cytotoxic T lymphocytes.

Conserved nucleotides in the TAR RNA stem of human immunodeficiency virus type 1 are critical for Tat binding and trans activation: model for TAR RNA tertiary structure.

Spleen necrosis virus, an avian immunosuppressive retrovirus, shares a receptor with the type D simian retroviruses.

Pseudorabies virus envelope glycoprotein gI influences both neurotropism and virulence during infection of the rat visual system.

Identification of T-helper epitopes in the VP1 capsid protein of poliovirus.

Identification and characterization of pseudorabies virus glycoprotein H.

Transactivation of erythroid transcription factor GATA-1 by a myb-ets-containing retrovirus.

Infection of HeLa cells with poliovirus results in modification of a complex that binds to the rRNA promoter.

Coding capacity determines in vivo accumulation of a defective RNA of clover yellow mosaic virus.

Expression of Aleutian mink disease parvovirus capsid proteins by a recombinant vaccinia virus: self-assembly of capsid proteins into particles.

Characterization of hepatitis B virus capsid particle assembly in Xenopus oocytes.

5-Azacytidine and RNA secondary structure increase the retrovirus mutation rate.

Specific interactions of HeLa cell proteins with proposed translation domains of the poliovirus 5' noncoding region.

Defective synthesis of early region 4 mRNAs during abortive adenovirus infections in monkey cells.

The pathogenesis of infection with minute virus of mice depends on expression of the small nonstructural protein NS2 and on the genotype of the allotropic determinants VP1 and VP2.

Mapping genetic determinants for human immunodeficiency virus type 1 resistance to soluble CD4.

The full-length transcript of a caulimovirus is a polycistronic mRNA whose genes are trans activated by the product of gene VI.

Nuclear factor I is specifically targeted to discrete subnuclear sites in adenovirus type 2-infected cells.

Mutation of human immunodeficiency virus type 1 at amino acid 585 on gp41 results in loss of killing by CD8+ A24-restricted cytotoxic T lymphocytes.

Viral infection of the thymus.

Poliovirus chimeras expressing sequences from the principal neutralization domain of human immunodeficiency virus type 1.

Evidence for zinc binding by two structural proteins of Plodia interpunctella granulosis virus.

Human papillomavirus type 33 in a tonsillar carcinoma generates its putative E7 mRNA via two E6* transcript species which are terminated at different early region poly(A) sites.

Reverse transcriptase of human immunodeficiency virus type 1: functionality of subunits of the heterodimer in DNA synthesis.

Phenotype-associated sequence variation in the third variable domain of the human immunodeficiency virus type 1 gp120 molecule.

Theiler's virus replication in brain macrophages cultured in vitro.

A mutation present in the amino terminus of Sabin 3 poliovirus VP1 protein is attenuating.

A block in release of progeny virus and a high particle-to-infectious unit ratio contribute to poor growth of enteric adenovirus types 40 and 41 in cell culture.

Human herpesvirus 7 is a constitutive inhabitant of adult human saliva.

A selectable marker allows investigation of a nontransforming Epstein-Barr virus mutant.

Replication of plasmid-derived human papillomavirus type 11 DNA in cultured keratinocytes.

Hepatitis C virus (HCV) circulates as a population of different but closely related genomes: quasispecies nature of HCV genome distribution.

Expression and extracellular release of human immunodeficiency virus type 1 Gag precursors by recombinant baculovirus-infected cells.

Transactivation of human immunodeficiency virus type 1 long terminal repeat-directed gene expression by the human foamy virus bel1 protein requires a specific DNA sequence.

Functional analysis of the true late human cytomegalovirus pp28 upstream promoter: cis-acting elements and viral trans-acting proteins necessary for promoter activation.

Transmembrane protein oligomers of caprine arthritis-encephalitis lentivirus are immunodominant in goats with progressive arthritis.

Cytolytic T lymphocytes specific for tumors and infected cells from mice with a retrovirus-induced immunodeficiency syndrome.

Consistent transcription of the Epstein-Barr virus LMP2 gene in nasopharyngeal carcinoma.

Nuclear entry and nucleolar localization of the Newcastle disease virus (NDV) matrix protein occur early in infection and do not require other NDV proteins.

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Hepatitis B virus (HBV)-specific cytotoxic T-cell response in humans: production of target cells by stable expression of HBV-encoded proteins in immortalized human B-cell lines.

Content Provider	PubMed Central
Author	Guilhot, S. Fowler, P. Portillo, G. Margolskee, R. F. Ferrari, C. Bertoletti, A. Chisari, F. V.
Abstract	To analyze the hepatitis B virus (HBV)-specific cytotoxic T-cell (CTL) response during acute and chronic viral hepatitis, target cells that express HBV-encoded antigens in the context of the appropriate HLA restriction element must be available for each subject studied. Since HBV is not infectious for human cells in vitro, such target cells must be produced by DNA-mediated gene transfer into cultured human primary cells or cell lines. For this purpose, we have developed a panel of Epstein-Barr virus-based episomal expression vectors containing each of the HBV open reading frames under the transcriptional control of the simian virus 40 early promoter. Transfection of Epstein-Barr virus-immortalized B-cell lines with this panel of recombinants consistently leads to stable expression of the HBV envelope, nucleocapsid, and polymerase proteins. The HBV X gene product is transiently expressed following transfection, but stable expression of this protein cannot be maintained on a long-term basis. To assess the suitability of this system for the identification of HBV-specific CTL in humans, a panel of EBO-HBV transfectants of defined HLA haplotype was used to monitor the HBV-specific CTL response in a patient with acute viral hepatitis type B. Transfectants that stably express the HBV nucleocapsid (core) antigen were found to serve as excellent targets for the detection of HLA class I-restricted CTL that recognize endogenously synthesized HBV core antigen in this patient; they were also successfully used to stimulate the specific expansion of these CTL in vitro.
Starting Page	2670
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	5
Volume Number	66
Language	English
Publisher Date	1992-05-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

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