NDLI: A 60-kDa plant microtubule-associated protein promotes the growth and stabilization of neurotubules in vitro

Content Provider	PubMed Central
Author	Rutten, Twan Chan, Jordi Lloyd, Clive W.
Copyright Year	1997
Abstract	The search for microtubule-associated proteins (MAPs) in plants is relatively recent. In particular, the “classical MAPs,” which stimulate the polymerization and stabilization of microtubules, have only been examined in heterogeneous fractions. As a first step in dissecting the role of individual MAPs, we have chromatographically purified a single 60-kDa protein from a carrot MAP fraction and analyzed its effects on tubulin assembly. MAP60 promoted the formation of long, morphologically regular brain microtubules in vitro, an effect inhibited by preincubation of the MAP with affinity-purified antibodies against this protein. MAP60 also increased the stability of microtubules to dilution and significantly enhanced cold stability to the normally cold-sensitive neurotubules. These in vitro properties are consistent with a role for MAP60 in regulating the turnover/assembly of dynamic plant microtubules in vivo.
Starting Page	4469
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	94
Language	English
Publisher	The National Academy of Sciences of the USA
Publisher Date	1997-04-29
Access Restriction	Open
Rights Holder	The National Academy of Sciences of the USA
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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A 60-kDa plant microtubule-associated protein promotes the growth and stabilization of neurotubules in vitro