NDLI: Organization of sister origins and replisomes during multifork DNA replication in Escherichia coli

Content Provider	PubMed Central
Author	Fossum, Solveig Crooke, Elliott Kirsten, Skarstad
Copyright Year	2007
Abstract	The replication period of Escherichia coli cells grown in rich medium lasts longer than one generation. Initiation thus occurs in the ‘mother-' or ‘grandmother generation'. Sister origins in such cells were found to be colocalized for an entire generation or more, whereas sister origins in slow-growing cells were colocalized for about 0.1–0.2 generations. The role of origin inactivation (sequestration) by the SeqA protein in origin colocalization was studied by comparing sequestration-deficient mutants with wild-type cells. Cells with mutant, non-sequesterable origins showed wild-type colocalization of sister origins. In contrast, cells unable to sequester new origins due to loss of SeqA, showed aberrant localization of origins indicating a lack of organization of new origins. In these cells, aberrant replisome organization was also found. These results suggest that correct organization of sister origins and sister replisomes is dependent on the binding of SeqA protein to newly formed DNA at the replication forks, but independent of origin sequestration. In agreement, in vitro experiments indicate that SeqA is capable of pairing newly replicated DNA molecules.
Related Links	http://dx.doi.org/10.1038/sj.emboj.7601871
Ending Page	4522
Page Count	9
Starting Page	4514
File Format	PDF
ISSN	02614189
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	21
Volume Number	26
Language	English
Publisher	Nature Publishing Group
Publisher Date	2007-10-31
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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