NDLI: Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice

Content Provider	PubMed Central
Author	Minowa, Osamu Arai, Tsuyoshi Hirano, Masanori Monden, Yoshiaki Nakai, Shigeyasu Fukuda, Mayuko Itoh, Masaki Takano, Hiroshi Yoshitaka, Hippou Hiroyuki, Aburatani Masumura, Ken-ichi Nohmi, Takehiko Nishimura, Susumu Noda, Tetsuo
Copyright Year	2000
Abstract	The major mutagenic base lesion in DNA caused by exposure to reactive oxygen species is 8-hydroxyguanine or 7,8-dihydro-8-oxoguanine (8-OH-G). Products of the human MMH/OGG1 gene are known to catalyze in vitro the reactions repairing this DNA lesion. To analyze the function of Mmh in vivo, we generated a mouse line carrying a mutant Mmh allele by targeted gene disruption. Mmh homozygous mutant mice were found to have a physically normal appearance, but to have lost nicking activity in liver extracts for substrate DNA containing 8-OH-G, exhibiting a 3-fold increased accumulation of this adduct at 9 weeks of age compared with wild-type or heterozygous mice. Further elevation to 7-fold was observed in 14-week-old animals. Substantial increase of spontaneous mutation frequencies was clearly identified in Mmh mutant mice bearing transgenic gpt genes. These results indicate that exposure of DNA to endogenous oxidative species continuously produces the mutagenic adduct 8-OH-G in mice, and Mmh plays an essential role in repair of this DNA damage.
Starting Page	4156
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	8
Volume Number	97
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2000-04-11
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice.

High accumulation of oxidative DNA damage, 8-hydroxyguanine, in Mmh/Ogg1 deficient mice by chronic oxidative stress

OGG1 protein suppresses G:C→T:A mutation in a shuttle vector containing 8-hydroxyguanine in human cells

Differential ability of polymorphic OGG1 proteins to suppress mutagenesis induced by 8-hydroxyguanine in human cell in vivo

8-oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity.

Changes in levels of 8-hydroxyguanine in DNA, its repair and OGG1 mRNA in rat lungs after intratracheal administration of diesel exhaust particles

Suppressive activities of OGG1 and MYH proteins against G:C to T:A mutations caused by 8-hydroxyguanine but not by benzo[a]pyrene diol epoxide in human cells in vivo

Genomic structure and chromosomal localization of the mouse Ogg1 gene that is involved in the repair of 8-hydroxyguanine in DNA damage

Repair of 8-hydroxyguanine in DNA by mammalian N-methylpurine-DNA glycosylase.

Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice

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Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice.

High accumulation of oxidative DNA damage, 8-hydroxyguanine, in Mmh/Ogg1 deficient mice by chronic oxidative stress

OGG1 protein suppresses G:C→T:A mutation in a shuttle vector containing 8-hydroxyguanine in human cells

Differential ability of polymorphic OGG1 proteins to suppress mutagenesis induced by 8-hydroxyguanine in human cell in vivo

8-oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity.

Changes in levels of 8-hydroxyguanine in DNA, its repair and OGG1 mRNA in rat lungs after intratracheal administration of diesel exhaust particles

Suppressive activities of OGG1 and MYH proteins against G:C to T:A mutations caused by 8-hydroxyguanine but not by benzo[a]pyrene diol epoxide in human cells in vivo

Genomic structure and chromosomal localization of the mouse Ogg1 gene that is involved in the repair of 8-hydroxyguanine in DNA damage

Repair of 8-hydroxyguanine in DNA by mammalian N-methylpurine-DNA glycosylase.

Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice