NDLI: SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage

Content Provider	PubMed Central
Author	Mao, Yong Sun, Mei Desai, Shyamal D. Liu, Leroy F.
Copyright Year	2000
Abstract	Ubiquitin/26S proteasome-dependent degradation of topoisomerase I (TOP1) has been suggested to be a unique repair response to TOP1-mediated DNA damage. In the current study, we show that treatment of mammalian cells or yeast cells expressing human DNA TOP1 with camptothecin (CPT) induces covalent modification of the TOP1 by SUMO-1/Smt3p, a ubiquitin-like protein. This conclusion is based on the following observations: (i) Mammalian DNA TOP1 conjugates induced by CPT were cross-reactive with SUMO-1/Smt3p-specific antibodies both in yeast expressing human DNA TOP1 as well as mammalian cells. (ii) The formation of TOP1 conjugates was shown to be dependent on UBC9, the E2 enzyme for SUMO-1/Smt3p. (iii) TOP1 physically interacts with UBC9. (iv) Ubc9 mutant yeast cells expressing human DNA TOP1 was hypersensitive to CPT, suggesting that UBC9/SUMO-1 may be involved in the repair of TOP1-mediated DNA damage.
Related Links	http://dx.doi.org/10.1073/pnas.080536597
Ending Page	4051
Page Count	6
Starting Page	4046
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	8
Volume Number	97
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2000-04-11
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage.

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SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage

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SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage.

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SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage