NDLI: In vitro cultivation of human islets from expanded ductal tissue

Content Provider	PubMed Central
Author	Susan, Bonner-weir Taneja, Monica Weir, Gordon C. Tatarkiewicz, Krystyna Song, Ki-ho Sharma, Arun O'neil, John J.
Copyright Year	2000
Abstract	A major obstacle to successful islet transplantation for both type 1 and 2 diabetes is an inadequate supply of insulin-producing tissue. This need for transplantable human islets has stimulated efforts to expand existing pancreatic islets and/or grow new ones. To test the hypothesis that human adult duct tissue could be expanded and differentiated in vitro to form islet cells, digested pancreatic tissue that is normally discarded from eight human islet isolations was cultured under conditions that allowed expansion of the ductal cells as a monolayer whereupon the cells were overlaid with a thin layer of Matrigel. With this manipulation, the monolayer of epithelial cells formed three-dimensional structures of ductal cysts from which 50-to 150-μm diameter islet-like clusters of pancreatic endocrine cells budded. Over 3–4 weeks culture the insulin content per flask increased 10- to 15-fold as the DNA content increased up to 7-fold. The cultivated human islet buds were shown by immunofluorescence to consist of cytokeratin 19-positive duct cells and hormone-positive islet cells. Double staining of insulin and non-β cell hormones in occasional cells indicated immature cells still in the process of differentiation. Insulin secretion studies were done over 24 h in culture. Compared with their basal secretion at 5 mM glucose, cysts/cultivated human islet buds exposed to stimulatory 20 mM glucose had a 2.3-fold increase in secreted insulin. Thus, duct tissue from human pancreas can be expanded in culture and then be directed to differentiate into glucose responsive islet tissue in vitro. This approach may provide a potential new source of pancreatic islet cells for transplantation.
Starting Page	7999
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	14
Volume Number	97
Language	English
Publisher	The National Academy of Sciences
Publisher Date	2000-07-05
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

In vitro cultivation of human islets from expanded ductal tissue.

In vitro cultivation of human fetal pancreatic ductal stem cells and their differentiation into insulin-producing cells

THE CULTIVATION OF HUMAN TISSUE IN VITRO

The Cultivation of Human Tumour Tissue in vitro

THE CULTIVATION OF HUMAN SARCOMATOUS TISSUE IN VITRO

Insulin release from human pancreatic islets in vitro

MiR-375 Promotes Redifferentiation of Adult Human β Cells Expanded In Vitro

C3d receptors are expressed on human monocytes after in vitro cultivation.

Islets from human donors with higher but not lower hemoglobin A1c levels respond to gastrin treatment in vitro

In vitro cultivation of human islets from expanded ductal tissue

Similar Documents

In vitro cultivation of human islets from expanded ductal tissue.

In vitro cultivation of human fetal pancreatic ductal stem cells and their differentiation into insulin-producing cells

THE CULTIVATION OF HUMAN TISSUE IN VITRO

The Cultivation of Human Tumour Tissue in vitro

THE CULTIVATION OF HUMAN SARCOMATOUS TISSUE IN VITRO

Insulin release from human pancreatic islets in vitro

MiR-375 Promotes Redifferentiation of Adult Human β Cells Expanded In Vitro

C3d receptors are expressed on human monocytes after in vitro cultivation.

Islets from human donors with higher but not lower hemoglobin A1c levels respond to gastrin treatment in vitro

In vitro cultivation of human islets from expanded ductal tissue