NDLI: Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine

Content Provider	PubMed Central
Author	Goto, Kazunori Matsuoka, Satoshi Noma, Akinori
Copyright Year	2004
Abstract	To explore the electrophysiological properties of the interstitial cells of Cajal (ICCs) and fibroblast-like cells (FLCs), we developed a new preparation by treating the murine small intestine with collagenase. This thin muscle layer preparation contained at least two types of interstitial cells around the enteric nerve bundles, and the cluster of smooth muscle cells displayed a rhythmic contraction. We morphologically identified ICCs and FLCs and conducted patch clamp experiments on each type of cell. The c-kit-positive CD34-negative ICCs showed spontaneous and rhythmic potential fluctuations, and a large transient inward current was evoked by depolarization under voltage clamp conditions. Once the inward current was triggered, it took a regenerative time course and lasted approximately 500 ms. The current was inactivated by continuous depolarization, and by removal of external Ca2+. The application of acetylcholine (ACh) prolonged the duration of spontaneous depolarization as well as the depolarization-induced inward current. This inward current showed a reversal potential of around +3 mV and was considered to be due to non-selective cation channels. The c-kit-negative CD34-positive FLCs showed irregular or regular potential fluctuations, and spontaneous outward current was observed under voltage clamp conditions. This outward current showed a reversal potential of around −80 mV and might be classified as a potassium current. We failed to observe major time- and voltage-dependent currents except the above two currents in the interstitial cells.
Related Links	http://dx.doi.org/10.1113/jphysiol.2004.063875
Ending Page	422
Page Count	12
Starting Page	411
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 2
Volume Number	559
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2004-09-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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