NDLI: A quantitative analysis of cell volume and resting potential determination and regulation in excitable cells

Content Provider	PubMed Central
Author	Fraser, James A. Huang, Christopher L-h
Copyright Year	2004
Abstract	This paper quantifies recent experimental results through a general physical description of the mechanisms that might control two fundamental cellular parameters, resting potential (E m) and cell volume (V c), thereby clarifying the complex relationships between them. E m was determined directly from a charge difference (CD) equation involving total intracellular ionic charge and membrane capacitance (C m). This avoided the equilibrium condition dE m/dt = 0 required in determinations of E m by previous work based on the Goldman-Hodgkin-Katz equation and its derivatives and thus permitted precise calculation of E m even under non-equilibrium conditions. It could accurately model the influence upon E m of changes in C m or V c and of membrane transport processes such as the Na+–K+-ATPase and ion cotransport. Given a stable and adequate membrane Na+–K+-ATPase density (N), V c and E m both converged to unique steady-state values even from sharply divergent initial intracellular ionic concentrations. For any constant set of transmembrane ion permeabilities, this set point of V c was then determined by the intracellular membrane-impermeant solute content (X− i) and its mean charge valency (z X), while in contrast, the set point of E m was determined solely by z X. Independent changes in membrane Na+ (P Na) or K+ permeabilities (P K) or activation of cation–chloride cotransporters could perturb V c and E m but subsequent reversal of such changes permitted full recovery of both V c and E m to the original set points. Proportionate changes in P Na, P K and N, or changes in Cl− permeability (P Cl) instead conserved steady-state V c and E m but altered their rates of relaxation following any discrete perturbation. P Cl additionally determined the relative effect of cotransporter activity on V c and E m, in agreement with recent experimental results. In contrast, changes in Xi − produced by introduction of a finite permeability term to X− (P X) that did not alter z X caused sustained changes in V c that were independent of E m and that persisted when P X returned to zero. Where such fluxes also altered the effective z X they additionally altered the steady state E m. This offers a basis for the suggested roles of amino acid fluxes in long-term volume regulatory processes in a variety of excitable tissues.
Related Links	http://dx.doi.org/10.1113/jphysiol.2004.065706
Ending Page	478
Page Count	20
Starting Page	459
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 2
Volume Number	559
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2004-09-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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