NDLI: The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3′ processing of U2 snRNA

Content Provider	PubMed Central
Author	Medlin, Joanne E. Uguen, Patricia Taylor, Alice Bentley, David L. Murphy, Shona
Copyright Year	2003
Abstract	The human snRNA genes transcribed by RNA polymerase II (e.g. U1 and U2) have a characteristic TATA-less promoter containing an essential proximal sequence element. Formation of the 3′ end of these non-polyadenylated RNAs requires a specialized 3′ box element whose function is promoter specific. Here we show that truncation of the C-terminal domain (CTD) of RNA polymerase II and treatment of cells with CTD kinase inhibitors, including DRB (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole), causes a dramatic reduction in proper 3′ end formation of U2 transcripts. Activation of 3′ box recognition by the phosphorylated CTD would be consistent with the role of phospho-CTD in mRNA processing. CTD kinase inhibitors, however, have little effect on initiation or elongation of transcription of the U2 genes, whereas elongation of transcription of the β-actin gene is severely affected. This result highlights differences in transcription of snRNA and mRNA genes.
Related Links	http://dx.doi.org/10.1093/emboj/cdg077
Ending Page	934
Page Count	10
Starting Page	925
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	4
Volume Number	22
Language	English
Publisher	Oxford University Press
Publisher Date	2003-02-17
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing.

Depletion of yeast RNase III blocks correct U2 3' end formation and results in polyadenylated but functional U2 snRNA.

A 10 residue motif at the C-terminus of the RNA pol II CTD is required for transcription, splicing and 3′ end processing

Transcription of the human U2 snRNA genes continues beyond the 3' box in vivo.

Role of the C-Terminal Domain of RNA Polymerase II in U2 snRNA Transcription and 3′ Processing

Role of the C-terminal domain of RNA polymerase II in U2 snRNA transcription and 3' processing.

All three SOS-inducible DNA polymerases (Pol II, Pol IV and Pol V) are involved in induced mutagenesis

U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP

Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis.

The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3′ processing of U2 snRNA

Similar Documents

Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing.

Depletion of yeast RNase III blocks correct U2 3' end formation and results in polyadenylated but functional U2 snRNA.

A 10 residue motif at the C-terminus of the RNA pol II CTD is required for transcription, splicing and 3′ end processing

Transcription of the human U2 snRNA genes continues beyond the 3' box in vivo.

Role of the C-Terminal Domain of RNA Polymerase II in U2 snRNA Transcription and 3′ Processing

Role of the C-terminal domain of RNA polymerase II in U2 snRNA transcription and 3' processing.

All three SOS-inducible DNA polymerases (Pol II, Pol IV and Pol V) are involved in induced mutagenesis

U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP

Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis.

The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3′ processing of U2 snRNA