NDLI: Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis.

Content Provider	PubMed Central
Author	Wendland, B. Steece, K. E. Emr, S. D.
Abstract	The mammalian protein epsin is required for endocytosis. In this study, we have characterized two homologous yeast proteins, Ent1p and Ent2p, which are similar to mammalian epsin. An essential function for the highly conserved N-terminal epsin N-terminal homology (ENTH) domain was revealed using deletions and randomly generated temperature-sensitive ent1 alleles. Changes in conserved ENTH domain residues in ent1(ts) cells revealed defects in endocytosis and actin cytoskeleton structure. The Ent1 protein was localized to peripheral and internal punctate structures, and biochemical fractionation studies found the protein associated with a large, Triton X-100-insoluble pellet. Finally, an Ent1p clathrin-binding domain was mapped to the final eight amino acids (RGYTLIDL*) in the Ent1 protein sequence. Based on these and other data, we propose that the yeast epsin-like proteins are essential components of an endocytic complex that may act at multiple stages in the endocytic pathway.
Related Links	http://dx.doi.org/10.1093/emboj/18.16.4383
Ending Page	4393
Page Count	11
Starting Page	4383
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	16
Volume Number	18
Language	English
Publisher Date	1999-08-16
Access Restriction	Open
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Clathrin-mediated endocytosis is essential in Trypanosoma brucei

Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation

Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain

An N-terminal nuclear export signal is required for the nucleocytoplasmic shuttling of IkappaBalpha.

The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3′ processing of U2 snRNA

Integrity of the N-terminal transcription domain of p53 is required for mutant p53 interference with drug-induced apoptosis

Ribosome biogenesis factors bind a nuclear envelope SUN domain protein to cluster yeast telomeres

The conserved N-terminal BH4 domain of Bcl-2 homologues is essential for inhibition of apoptosis and interaction with CED-4.

Two elements target SIV Nef to the AP-2 clathrin adaptor complex, but only one is required for the induction of CD4 endocytosis.

Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis.

Similar Documents

Clathrin-mediated endocytosis is essential in Trypanosoma brucei

Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation

Regulation of clathrin adaptor function in endocytosis: novel role for the SAM domain

An N-terminal nuclear export signal is required for the nucleocytoplasmic shuttling of IkappaBalpha.

The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3′ processing of U2 snRNA

Integrity of the N-terminal transcription domain of p53 is required for mutant p53 interference with drug-induced apoptosis

Ribosome biogenesis factors bind a nuclear envelope SUN domain protein to cluster yeast telomeres

The conserved N-terminal BH4 domain of Bcl-2 homologues is essential for inhibition of apoptosis and interaction with CED-4.

Two elements target SIV Nef to the AP-2 clathrin adaptor complex, but only one is required for the induction of CD4 endocytosis.

Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis.