NDLI: Pressure Equilibrium and Jump Study on Unfolding of 23-kDa Protein from Spinach Photosystem II

Content Provider	PubMed Central
Author	Tan, Cui-yan Xu, Chun-he Wong, Jun Shen, Jian-ren Sakuma, Shinsuke Yamamoto, Yasusi Lange, Reinhard Claude, Balny Ruan, Kang-cheng
Copyright Year	2005
Abstract	Pressure-induced unfolding of 23-kDa protein from spinach photosystem II has been systematically investigated at various experimental conditions. Thermodynamic equilibrium studies indicate that the protein is very sensitive to pressure. At 20°C and pH 5.5, 23-kDa protein shows a reversible two-state unfolding transition under pressure with a midpoint near 160 MPa, which is much lower than most natural proteins studied to date. The free energy (ΔG u) and volume change (ΔV u) for the unfolding are 5.9 kcal/mol and −160 ml/mol, respectively. It was found that NaCl and sucrose significantly stabilize the protein from unfolding and the stabilization is associated not only with an increase in ΔG u but also with a decrease in ΔV u. The pressure-jump studies of 23-kDa protein reveal a negative activation volume for unfolding (−66.2 ml/mol) and a positive activation volume for refolding (84.1 ml/mol), indicating that, in terms of system volume, the protein transition state lies between the folded and unfolded states. Examination of the temperature effect on the unfolding kinetics indicates that the thermal expansibility of the transition state and the unfolded state of 23-kDa protein are closer to each other and they are larger than that of the native state. The diverse pressure-refolding pathways of 23-kDa protein in some conditions were revealed in pressure-jump kinetics.
Related Links	http://dx.doi.org/10.1529/biophysj.104.050435
Starting Page	1264
File Format	PDF
ISSN	15420086
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	2
Volume Number	88
Language	English
Publisher	Biophysical Society
Publisher Date	2005-02-01
Access Restriction	Open
Rights Holder	Biophysical Society
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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