NDLI: Control of the Expression and Compartmentalization of σG Activity during Sporulation of Bacillus subtilis by Regulators of σF and σE

Content Provider	PubMed Central
Author	Chary, Vasant K. Meloni, Mauro Hilbert, David W. Piggot, Patrick J.
Copyright Year	2005
Abstract	During formation of spores by Bacillus subtilis the RNA polymerase factor σG ordinarily becomes active during spore formation exclusively in the prespore upon completion of engulfment of the prespore by the mother cell. Formation and activation of σG ordinarily requires prior activity of σF in the prespore and σE in the mother cell. Here we report that in spoIIA mutants lacking both σF and the anti-sigma factor SpoIIAB and in which σE is not active, σG nevertheless becomes active. Further, its activity is largely confined to the mother cell. Thus, there is a switch in the location of σG activity from prespore to mother cell. Factors contributing to the mother cell location are inferred to be read-through of spoIIIG, the structural gene for σG, from the upstream spoIIG locus and the absence of SpoIIAB, which can act in the mother cell as an anti-sigma factor to σG. When the spoIIIG locus was moved away from spoIIG to the distal amyE locus, σG became active earlier in sporulation in spoIIA deletion mutants, and the sporulation septum was not formed, suggesting that premature σG activation can block septum formation. We report a previously unrecognized control in which SpoIIGA can prevent the appearance of σG activity, and pro-σE (but not σE) can counteract this effect of SpoIIGA. We find that in strains lacking σF and SpoIIAB and engineered to produce active σE in the mother cell without the need for SpoIIGA, σG also becomes active in the mother cell.
Related Links	http://dx.doi.org/10.1128/jb.187.19.6832-6840.2005
Ending Page	6840
Page Count	9
Starting Page	6832
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	19
Volume Number	187
Language	English
Publisher	American Society for Microbiology
Publisher Date	2005-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Contrasting Effects of σE on Compartmentalization of σF Activity during Sporulation of Bacillus subtilis

Expression of the σF-Directed csfB Locus Prevents Premature Appearance of σG Activity during Sporulation of Bacillus subtilis ▿

A Small Protein Required for the Switch from σF to σG during Sporulation in Bacillus subtilis ▿

SpoIIE Is Necessary for Asymmetric Division, Sporulation, and Expression of σF, σE, and σG but Does Not Control Solvent Production in Clostridium acetobutylicum ATCC 824 ▿ ‡

Blocking Chromosome Translocation during Sporulation of Bacillus subtilis Can Result in Prespore-Specific Activation of σG That Is Independent of σE and of Engulfment

Inactivation of σF in Clostridium acetobutylicum ATCC 824 Blocks Sporulation Prior to Asymmetric Division and Abolishes σE and σG Protein Expression but Does Not Block Solvent Formation ▿ †

Role of the Anti-Sigma Factor SpoIIAB in Regulation of σG during Bacillus subtilis Sporulation

Loss of Compartmentalization of σE Activity Need Not Prevent Formation of Spores by Bacillus subtilis ▿ †

Compartmentalization of Gene Expression during Sporulation of Bacillus subtilis Is Compromised in Mutants Blocked at Stage III of Sporulation

Control of the Expression and Compartmentalization of σG Activity during Sporulation of Bacillus subtilis by Regulators of σF and σE

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