NDLI: Inactivation of σF in Clostridium acetobutylicum ATCC 824 Blocks Sporulation Prior to Asymmetric Division and Abolishes σE and σG Protein Expression but Does Not Block Solvent Formation ▿ †

Content Provider	PubMed Central
Author	Jones, Shawn W. Tracy, Bryan P. Gaida, Stefan M. Papoutsakis, Eleftherios T.
Copyright Year	2011
Abstract	Clostridium acetobutylicum is both a model organism for the understanding of sporulation in solventogenic clostridia and its relationship to solvent formation and an industrial organism for anaerobic acetone-butanol-ethanol (ABE) fermentation. How solvent production is coupled to endospore formation—both stationary-phase events—remains incompletely understood at the molecular level. Specifically, it is unclear how sporulation-specific sigma factors affect solvent formation. Here the sigF gene in C. acetobutylicum was successfully disrupted and silenced. Not only σF but also the sigma factors σE and σG were not detected in the sigF mutant (FKO1), and differentiation was stopped prior to asymmetric division. Since plasmid expression of the spoIIA operon (spoIIAA-spoIIAB-sigF) failed to complement FKO1, the operon was integrated into the FKO1 chromosome to generate strain FKO1-C. In FKO1-C, σF expression was restored along with sporulation and σE and σG protein expression. Quantitative reverse transcription-PCR (RT-PCR) analysis of a select set of genes (csfB, gpr, spoIIP, sigG, lonB, and spoIIR) that could be controlled by σF, based on the Bacillus subtilis model, indicated that sigG may be under the control of σF, but spoIIR, an important activator of σE in B. subtilis, is not, and neither are the rest of the genes investigated. FKO1 produced solvents at a level similar to that of the parent strain, but solvent levels were dependent on the physiological state of the inoculum. Finally, the complementation strain FKO1-C is the first reported instance of purposeful integration of multiple functional genes into a clostridial chromosome—here, the C. acetobutylicum chromosome—with the aim of altering cell metabolism and differentiation.
Related Links	http://dx.doi.org/10.1128/jb.00088-11
Ending Page	2440
Page Count	12
Starting Page	2429
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	10
Volume Number	193
Language	English
Publisher	American Society for Microbiology
Publisher Date	2011-05-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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SpoIIE Is Necessary for Asymmetric Division, Sporulation, and Expression of σF, σE, and σG but Does Not Control Solvent Production in Clostridium acetobutylicum ATCC 824 ▿ ‡

Control of the Expression and Compartmentalization of σG Activity during Sporulation of Bacillus subtilis by Regulators of σF and σE

Inactivation of σE and σG in Clostridium acetobutylicum Illuminates Their Roles in Clostridial-Cell-Form Biogenesis, Granulose Synthesis, Solventogenesis, and Spore Morphogenesis ▿ †

Northern, Morphological, and Fermentation Analysis of spo0A Inactivation and Overexpression in Clostridium acetobutylicum ATCC 824

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Physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome.

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Inactivation of σF in Clostridium acetobutylicum ATCC 824 Blocks Sporulation Prior to Asymmetric Division and Abolishes σE and σG Protein Expression but Does Not Block Solvent Formation ▿ †

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