NDLI: Small Untranslated RNA Antitoxin in Bacillus subtilis †

Content Provider	PubMed Central
Author	Silvaggi, Jessica M. Perkins, John B. Losick, Richard
Copyright Year	2005
Abstract	Toxin-antitoxin (TA) modules are pairs of genes in which one member encodes a toxin that is neutralized or whose synthesis is prevented by the action of the product of the second gene, an antitoxin, which is either protein or RNA. We now report the identification of a TA module in the chromosome of Bacillus subtilis in which the antitoxin is an antisense RNA. The antitoxin, which is called RatA (for RNA antitoxin A), is a small (222 nucleotides), untranslated RNA that blocks the accumulation of the mRNA for a toxic peptide TxpA (for toxic peptide A; formerly YqdB). The txpA and ratA genes are in convergent orientation and overlap by ca. 75 nucleotides, such that the 3′ region of ratA is complementary to the 3′ region of txpA. Deletion of ratA led to increased levels of txpA mRNA and lysis of the cells. Overexpression of txpA also caused cell lysis and death, a phenotype that was prevented by simultaneous overexpression of ratA. We propose that the ratA transcript is an antisense RNA that anneals to the 3′ end of the txpA mRNA, thereby triggering its degradation.
Related Links	http://dx.doi.org/10.1128/jb.187.19.6641-6650.2005
Ending Page	6650
Page Count	10
Starting Page	6641
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	19
Volume Number	187
Language	English
Publisher	American Society for Microbiology
Publisher Date	2005-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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