NDLI: Genetic analysis of the archaeon Methanosarcinabarkeri Fusaro reveals a central role for Ech hydrogenaseand ferredoxin in methanogenesis and carbon fixation

Content Provider	PubMed Central
Author	Meuer, Jörn Kuettner, H. Craig Zhang, Jun Kai Hedderich, Reiner Metcalf, William W.
Copyright Year	2002
Abstract	Ech hydrogenase (Ech) from the methanogenic archaeonMethanosarcina barkeri catalyzes the reversiblereduction of ferredoxin by H2 and is a member of a distinctgroup of membrane-bound [NiFe] hydrogenases with sequence similarityto energy-conserving NADH:quinone oxidoreductase (complex I). Toelucidate the physiological role(s) of Ech a mutant lacking thisenzyme was constructed. The mutant was unable to grow onmethanol/H2/CO2,H2/CO2, or acetate as carbon and energysources but showed wild-type growth rates with methanol as solesubstrate. Addition of pyruvate to the growth medium restored growth onmethanol/H2/CO2 but not onH2/CO2 or acetate. Results obtained fromgrowth experiments, cell suspension experiments, and enzyme activitymeasurements in cell extracts provide compelling evidence for essentialfunctions of Ech and a 2[4Fe-4S] ferredoxin in the metabolism ofM. barkeri. The following conclusions were made.(i) In acetoclastic methanogenesis, Ech catalyzesH2 formation from reduced ferredoxin, generated by theoxidation of the carbonyl group of acetate to CO2.(ii) Under autotrophic growth conditions, the enzymecatalyzes the energetically unfavorable reduction of ferredoxin byH2, most probably driven by reversed electron transport,and the reduced ferredoxin thus generated functions as low potentialelectron donor for the synthesis of pyruvate in an anabolic pathway.(iii) Reduced ferredoxin in addition provides thereducing equivalents for the first step of methanogenesis fromH2/CO2, the reduction of CO2 toformylmethanofuran. Thus, in vivo genetic analysis hasled to the identification of the electron donor of this keyinitial step of methanogenesis.
Related Links	http://dx.doi.org/10.1073/pnas.072615499
Ending Page	5637
Page Count	6
Starting Page	5632
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	8
Volume Number	99
Language	English
Publisher	National Academy of Sciences
Publisher Date	2002-04-16
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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