NDLI: Human antibodies against spores of the genusBacillus: A model study for detection of and protectionagainst anthrax and the bioterrorist threat

Content Provider	PubMed Central
Author	Zhou, Bin Wirsching, Peter Janda, Kim D.
Copyright Year	2002
Abstract	A naïve, human single-chain Fv (scFv) phage-displaylibrary was used in bio-panning against live, native spores ofBacillus subtilis IFO 3336 suspended in solution. Adirect in vitro panning and enzyme-linked immunosorbentassay-based selection afforded a panel of nine scFv-phage clones ofwhich two, 5B and 7E, were chosen for further study. These two clonesdiffered in their relative specificity and affinity for spores ofB. subtilis IFO 3336 vs. a panel of spores from 11 otherBacillus species/strains. A variety of enzyme-linkedimmunosorbent assay protocols indicated these scFv-phage clonesrecognized different spore epitopes. Notably, some spore epitopesmarkedly changed between the free and microtiter-plate immobilizedstate as revealed by antibody-phage binding. An additional libraryselection procedure also was examined by constructing a Fabchain-shuffled sublibrary from the nine positive clones and by using asubtractive panning strategy to remove crossreactivity with B.licheniformis 5A24. The Fab-phage clone 52 was improvedcompared with 5B and was comparable to 7E in binding B.subtilis IFO 3336 vs. B. licheniformis 5A24, yetshowed a distinctive crossreactivity pattern with other spores. We alsodeveloped a method to directly detect individual spores by usingfluorescently labeled antibody-phage. Finally, a variety of“powders” that might be used in deploying spores of B.anthracis were examined for antibody-phage binding. Thestrategies described provide a foundation to discover human antibodiesspecific for native spores of B. anthracis that can bedeveloped as diagnostic and therapeutic reagents.
Related Links	http://dx.doi.org/10.1073/pnas.082121599
Starting Page	5241
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	8
Volume Number	99
Language	English
Publisher	National Academy of Sciences
Publisher Date	2002-04-16
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Human antibodies against spores of the genus Bacillus: a model study for detection of and protection against anthrax and the bioterrorist threat.

A cationic lipid-formulated plasmid DNA vaccine confers sustained antibody-mediated protection against aerosolized anthrax spores

A cationic lipid-formulated plasmid DNA vaccine confers sustained antibody-mediated protection against aerosolized anthrax spores.

Development of Antibodies against Anthrose Tetrasaccharide for Specific Detection of Bacillus anthracis Spores▿

The Detection of Anthrax Spores in Industrial Material

A Novel Blocking ELISA for Detection of Antibodies against Hepatitis E Virus in Domestic Pigs

Efficacy of a Vaccine Based on Protective Antigen and Killed Spores against Experimental Inhalational Anthrax▿ ‡

Germicidal Efficiency of Chlorine and the N-chloro Derivatives of Ammonia, Methylamine and Glycine Against Anthrax Spores

Study of antibodies against human melanoma produced by somatic cell hybrids.

Human antibodies against spores of the genusBacillus: A model study for detection of and protectionagainst anthrax and the bioterrorist threat

Similar Documents

Human antibodies against spores of the genus Bacillus: a model study for detection of and protection against anthrax and the bioterrorist threat.

A cationic lipid-formulated plasmid DNA vaccine confers sustained antibody-mediated protection against aerosolized anthrax spores

A cationic lipid-formulated plasmid DNA vaccine confers sustained antibody-mediated protection against aerosolized anthrax spores.

Development of Antibodies against Anthrose Tetrasaccharide for Specific Detection of Bacillus anthracis Spores▿

The Detection of Anthrax Spores in Industrial Material

A Novel Blocking ELISA for Detection of Antibodies against Hepatitis E Virus in Domestic Pigs

Efficacy of a Vaccine Based on Protective Antigen and Killed Spores against Experimental Inhalational Anthrax▿ ‡

Germicidal Efficiency of Chlorine and the N-chloro Derivatives of Ammonia, Methylamine and Glycine Against Anthrax Spores

Study of antibodies against human melanoma produced by somatic cell hybrids.

Human antibodies against spores of the genusBacillus: A model study for detection of and protectionagainst anthrax and the bioterrorist threat