NDLI: Rapid induction of uterine tumors with p53 point mutations in heterozygous p53-deficient CBA mice given a single intraperitoneal administration of N-ethyl-N-nitrosourea

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Rapid induction of uterine tumors with p53 point mutations in heterozygous p53-deficient CBA mice given a single intraperitoneal administration of N-ethyl-N-nitrosourea

Content Provider	Paperity
Author	Takagi, Hisayoshi Mitsumori, Kunitoshi Yasuhara, Kazuo Koujitani, Takatoshi Wakana, Shigeharu Hirose, Masao Onodera, Hiroshi Maruyama, Chika Shimo, Takeo
Abstract	To investigate the sensitivity of heterozygous p53-deficient CBA mice to carcinogens, 20 female mice [p53(+/–)] and 20 wild-type littermates [p53(+/+)] were given an intraperitoneal injection of 120 mg/kg body wt of N-ethyl-N-nitrosourea (ENU) and were maintained without any other treatment for a further 26 weeks. Histopathology showed that uterine tumors (endometrial polyps and stromal sarcomas) and lung adenomas were induced in both p53(+/–) and p53(+/+) mice. The incidence of uterine tumors and lung adenomas (94% and 81%, respectively) in p53(+/–) mice was significantly greater than that in p53 (+/+) mice (37% and 42%, respectively). Malignant lymphomas were only induced in p53(+/–) mice, at an incidence of 31%. Concerning uterine tumors and preneoplastic lesions, there were endometrial stromal sarcomas and atypical hyperplasias of the endometrial gland in 90% and 63%, respectively, of p53(+/–) mice, with significantly greater incidences than in p53(+/+) mice. Gene analysis revealed GCG→GTG point mutations in codon 135 of exon 5 of the p53 allele in all of the uterine endometrial stromal sarcomas examined. Our results suggest that female p53(+/–) CBA mice are very susceptible to uterine carcinogenesis, providing a useful model for ENU-induced uterine tumors.
Starting Page	1039
Ending Page	1042
File Format	HTM / HTML
ISSN	01433334
DOI	10.1093/carcin/21.5.1039
Issue Number	5
Journal	Carcinogenesis
Volume Number	21
e-ISSN	14602180
Language	English
Publisher	Oxford University Press
Publisher Date	2000-05-01
Access Restriction	Open
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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