NDLI: Test No. 427: Skin Absorption: In Vivo Method

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Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene

Test No. 491: Short Time Exposure In Vitro Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage

Test No. 492: Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage

Test No. 493: Performance-Based Test Guideline for Human Recombinant Estrogen Receptor (hrER) In Vitro Assays to Detect Chemicals with ER Binding Affinity

Test No. 404: Acute Dermal Irritation/Corrosion

Test No. 421: Reproduction/Developmental Toxicity Screening Test

Test No. 422: Combined Repeated Dose Toxicity Study with the Reproduction/Developmental Toxicity Screening Test

Test No. 430: In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test Method (TER)

Test No. 431: In vitro skin corrosion: reconstructed human epidermis (RHE) test method

Test No. 435: In Vitro Membrane Barrier Test Method for Skin Corrosion

Test No. 439: In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method

Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists

Test No. 476: In Vitro Mammalian Cell Gene Mutation Tests using the Hprt and xprt genes

Test No. 478: Rodent Dominant Lethal Test

Test No. 483: Mammalian Spermatogonial Chromosomal Aberration Test

Test No. 442C: In Chemico Skin Sensitisation. Direct Peptide Reactivity Assay (DPRA).

Test No. 442D: In Vitro Skin Sensitisation. ARE-Nrf2 Luciferase Test Method.

Test No. 489: In Vivo Mammalian Alkaline Comet Assay

Test No. 473: In Vitro Mammalian Chromosomal Aberration Test

Test No. 474: Mammalian Erythrocyte Micronucleus Test

Test No. 475: Mammalian Bone Marrow Chromosomal Aberration Test

Test No. 487: In Vitro Mammalian Cell Micronucleus Test

Test No. 437: Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage

Test No. 438: Isolated Chicken Eye Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage

Test No. 439: In Vitro Skin Irritation - Reconstructed Human Epidermis Test Method

Test No. 488: Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays

Test No. 405: Acute Eye Irritation/Corrosion

Test No. 443: Extended One-Generation Reproductive Toxicity Study

Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists

Test No. 457: BG1Luc Estrogen Receptor Transactivation Test Method for Identifying Estrogen Receptor Agonists and Antagonists

Test No. 460: Fluorescein Leakage Test Method for Identifying Ocular Corrosives and Severe Irritants

Test No. 456: H295R Steroidogenesis Assay

Test No. 417: Toxicokinetics

Test No. 429: Skin Sensitisation. Local Lymph Node Assay.

Test No. 439: In Vitro Skin Irritation. Reconstructed Human Epidermis Test Method.

Test No. 442A: Skin Sensitization. Local Lymph Node Assay: DA.

Test No. 442B: Skin Sensitization. Local Lymph Node Assay: BrdU-ELISA.

Test No. 403: Acute Inhalation Toxicity

Test No. 412: Subacute Inhalation Toxicity: 28-Day Study

Test No. 413: Subchronic Inhalation Toxicity: 90-day Study

Test No. 451: Carcinogenicity Studies

Test No. 452: Chronic Toxicity Studies

Test No. 453: Combined Chronic Toxicity/Carcinogenicity Studies

Test No. 436: Acute Inhalation Toxicity – Acute Toxic Class Method

Test No. 437: Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants

Test No. 438: Isolated Chicken Eye Test Method for Identifying Ocular Corrosives and Severe Irritants

Test No. 441: Hershberger Bioassay in Rats. A Short-term Screening Assay for (Anti)Androgenic Properties.

Test No. 455: The Stably Transfected Human Estrogen Receptor-alpha Transcriptional Activation Assay for Detection of Estrogenic Agonist-Activity of Chemicals

Test No. 407: Repeated Dose 28-day Oral Toxicity Study in Rodents

Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure

Test No. 426: Developmental Neurotoxicity Study

Test No. 440: Uterotrophic Bioassay in Rodents. A short-term screening test for oestrogenic properties.

Summary of Considerations in the Report from the OECD Expert Groups on Short Term and Long Term Toxicology

Test No. 427: Skin Absorption: In Vivo Method

Test No. 428: Skin Absorption: In Vitro Method

Test No. 430: In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test (TER)

Test No. 431: In Vitro Skin Corrosion: Human Skin Model Test

Test No. 432: In Vitro 3T3 NRU Phototoxicity Test

Test No. 420: Acute Oral Toxicity - Fixed Dose Procedure

Test No. 423: Acute Oral toxicity - Acute Toxic Class Method

Test No. 414: Prenatal Development Toxicity Study

Test No. 416: Two-Generation Reproduction Toxicity

Test No. 408: Repeated Dose 90-Day Oral Toxicity Study in Rodents

Test No. 409: Repeated Dose 90-Day Oral Toxicity Study in Non-Rodents

Test No. 424: Neurotoxicity Study in Rodents

Test No. 471: Bacterial Reverse Mutation Test

Test No. 473: In vitro Mammalian Chromosome Aberration Test

Test No. 475: Mammalian Bone Marrow Chromosome Aberration Test

Test No. 476: In vitro Mammalian Cell Gene Mutation Test

Test No. 483: Mammalian Spermatogonial Chromosome Aberration Test

Test No. 486: Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo

Test No. 418: Delayed Neurotoxicity of Organophosphorus Substances Following Acute Exposure

Test No. 419: Delayed Neurotoxicity of Organophosphorus Substances: 28-day Repeated Dose Study

Test No. 406: Skin Sensitisation

Test No. 401: Acute Oral Toxicity

Test No. 402: Acute Dermal Toxicity

Test No. 479: Genetic Toxicology: In vitro Sister Chromatid Exchange Assay in Mammalian Cells

Test No. 480: Genetic Toxicology: Saccharomyces cerevisiae, Gene Mutation Assay

Test No. 481: Genetic Toxicology: Saacharomyces cerevisiae, Miotic Recombination Assay

Test No. 482: Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells in vitro

Test No. 484: Genetic Toxicology: Mouse Spot Test

Test No. 485: Genetic toxicology, Mouse Heritable Translocation Assay

Test No. 477: Genetic Toxicology: Sex-Linked Recessive Lethal Test in Drosophila melanogaster

Test No. 478: Genetic Toxicology: Rodent Dominant Lethal Test

Test No. 415: One-Generation Reproduction Toxicity Study

Test No. 410: Repeated Dose Dermal Toxicity: 21/28-day Study

Test No. 411: Subchronic Dermal Toxicity: 90-day Study

Test No. 427: Skin Absorption: In Vivo Method

Content Provider	OECD iLibrary
Organization	OECD
Abstract	The in vivo percutaneous absorption study set out in this Test Guideline provides the linkage necessary to extrapolate from oral studies when making safety assessments following dermal exposure. The in vivo method, described in this guideline, allows the determination of the penetration of the test substance through the skin into the systemic compartment. The test substance, preferably radiolabelled, is applied, for a fixed period of time, to the clipped skin of animals at one or more appropriate dose levels in the form of a representative in-use preparation. The rat is the most commonly used species. At least four animals of one sex should be used for each test preparation and each scheduled termination time. A known amount of the test preparation is evenly applied to the site. This amount should normally mimic potential human exposure, typically 1-5 mg/cm² for a solid or up to 10 µl/cm² for liquids. A relevant exposure period (typically 6 or 24 hours) should be used, based on the expected human exposure duration. The animals should be observed for signs of toxicity/abnormal reactions at intervals for the entire duration of the study. This study includes: daily measurements (excreta), regular detailed observations, as well as sacrifice at the scheduled time and blood collected for analysis.
Page Count	8
ISSN	20745788
e-ISBN	9789264071063
Language	English
Publisher	OECD Publishing
Publisher Date	2004-11-23
Access Restriction	Open
Subject Keyword	Environment
Content Type	Text
Resource Type	Book

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