NDLI: Mitochondrial $Ca^{2+}$ Dynamics in MCU Knockout C. elegans Worms

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Mitochondrial $Ca^{2+}$ Dynamics in MCU Knockout C. elegans Worms

Content Provider	MDPI
Author	Pilar, Álvarez-Illera Paloma, García-Casas Fonteriz, Rosalba I. Montero, Mayte Alvarez, Javier
Copyright Year	2020
Description	Mitochondrial [Ca2+] plays an important role in the regulation of mitochondrial function, controlling ATP production and apoptosis triggered by mitochondrial Ca2+ overload. This regulation depends on Ca2+ entry into the mitochondria during cell activation processes, which is thought to occur through the mitochondrial Ca2+ uniporter (MCU). Here, we have studied the mitochondrial Ca2+ dynamics in control and MCU-defective C. elegans worms in vivo, by using worms expressing mitochondrially-targeted YC3.60 yellow cameleon in pharynx muscle. Our data show that the small mitochondrial Ca2+ oscillations that occur during normal physiological activity of the pharynx were very similar in both control and MCU-defective worms, except for some kinetic differences that could mostly be explained by changes in neuronal stimulation of the pharynx. However, direct pharynx muscle stimulation with carbachol triggered a large and prolonged increase in mitochondrial [Ca2+] that was much larger in control worms than in MCU-defective worms. This suggests that MCU is necessary for the fast mitochondrial Ca2+ uptake induced by large cell stimulations. However, low-amplitude mitochondrial Ca2+ oscillations occurring under more physiological conditions are independent of the MCU and use a different Ca2+ pathway.
Starting Page	8622
e-ISSN	14220067
DOI	10.3390/ijms21228622
Journal	International Journal of Molecular Sciences
Issue Number	22
Volume Number	21
Language	English
Publisher	MDPI
Publisher Date	2020-11-16
Access Restriction	Open
Subject Keyword	International Journal of Molecular Sciences Developmental Biology C. Elegans Mitochondria Mitochondrial Calcium Uniporter Mcu Knockout Calcium Dynamics
Content Type	Text
Resource Type	Article

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