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Synthesis of Deuterium-Labeled Vitamin D Metabolites as Internal Standards for LC-MS Analysis
| Content Provider | MDPI |
|---|---|
| Author | Nagata, Akiko Iijima, Kazuto Sakamoto, Ryota Mizumoto, Yuka Iwaki, Miho Takiwaki, Masaki Kikutani, Yoshikuni Fukuzawa, Seketsu Odagi, Minami Tera, Masayuki Nagasawa, Kazuo |
| Copyright Year | 2022 |
| Description | Blood levels of the vitamin $D_{3}$ $(D_{3}$) metabolites 25-hydroxyvitamin $D_{3}$ $(25(OH)D_{3}$), 24R,25-dihydroxyvitamin $D_{3}$, and 1α,25-dihydroxyvitamin $D_{3}$ $(1,25(OH)_{2}D_{3}$) are recognized indicators for the diagnosis of bone metabolism-related diseases, $D_{3}$ deficiency-related diseases, and hypercalcemia, and are generally measured by liquid-chromatography tandem mass spectrometry (LC-MS/MS) using an isotope dilution method. However, other $D_{3}$ metabolites, such as 20-hydroxyvitamin $D_{3}$ and lactone $D_{3}$, also show interesting biological activities and stable isotope-labeled derivatives are required for LC-MS/MS analysis of their concentrations in serum. Here, we describe a versatile synthesis of deuterium-labeled $D_{3}$ metabolites using A-ring synthons containing three deuterium atoms. Deuterium-labeled $25(OH)D_{3}$ (2), $25(OH)D_{3}$-23,26-lactone (6), and $1,25(OH)_{2}D_{3}$-23,26-lactone (7) were synthesized, and successfully applied as internal standards for the measurement of these compounds in pooled human serum. This is the first quantification of $1,25(OH)_{2}D_{3}$-23,26-lactone (7) in human serum. |
| Starting Page | 2427 |
| e-ISSN | 14203049 |
| DOI | 10.3390/molecules27082427 |
| Journal | Molecules |
| Issue Number | 8 |
| Volume Number | 27 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2022-04-09 |
| Access Restriction | Open |
| Subject Keyword | Molecules Biochemical Research Vitamin D Deuterium Labeling Liquid-chromatography Tandem Mass Spectrometry Measurement of Vitamin D Metabolites in Blood |
| Content Type | Text |
| Resource Type | Article |
