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A Novel Method for the Determination of Vitamin D Metabolites Assessed at the Blood-Cerebrospinal Fluid Barrier
| Content Provider | MDPI |
|---|---|
| Author | Zelzer, Sieglinde Meinitzer, Andreas Herrmann, Markus Goessler, Walter Enko, Dietmar |
| Copyright Year | 2021 |
| Description | The brain’s supply with vitamin D is poorly understood. Therefore, the present study aimed to determine 25-hydroxy vitamin D3 (25(OH)D) and 24,25-dihydroxy vitamin D $(24,25(OH)_{2}D_{3}$) in serum and cerebrospinal fluid (CSF) from individuals with intact and disturbed brain-CSF-barrier (BCB) function. In 292 pairs of serum and CSF samples the vitamin D metabolites were measured with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). CSF/serum ratios $(Q_{ALB}$, $Q_{25(OH)D}$, $Q_{24,25(OH)2D3}$) were calculated. Median (IQR) serum concentrations of 25(OH)D and $24,25(OH)_{2}D_{3}$ were 63.8 (43.4–83.9) nmol/L and 4.2 (2.2–6.2) nmol/L. The CSF concentrations of both metabolites accounted for 3.7 and 3.3% of the respective serum concentrations. Serum 25(OH)D correlated inversely with $Q_{25(OH)D}$ and $Q_{24,25(OH)2D3}$ implying a more efficient transport of both metabolites across the BCB when the serum concentration of 25(OH)D is low. In patients with BCB dysfunction, the CSF concentrations and the CSF/serum ratios of both vitamin D metabolites were higher than in individuals with intact BCB. The CSF concentrations of 25(OH)D and $24,25(OH)_{2}D_{3}$ depend on BCB function and the respective serum concentrations of both metabolites. Higher vitamin D metabolite concentrations in CSF of patients with impaired BCB function may be due to passive diffusion across the BCB. |
| Starting Page | 1288 |
| e-ISSN | 2218273X |
| DOI | 10.3390/biom11091288 |
| Journal | Biomolecules |
| Issue Number | 9 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-08-29 |
| Access Restriction | Open |
| Subject Keyword | Biomolecules Biochemical Research Vitamin D Metabolites Cerebrospinal Fluid Blood-cerebrospinal Fluid Barrier Liquid Chromatography-tandem Mass Spectrometry Biomarkers |
| Content Type | Text |
| Resource Type | Article |
