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Isomer Conversion and Other Effects on Accuracy of Vitamin D Measurement by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Huang, Min Winters, Doug |
| Copyright Year | 2011 |
| Abstract | The accurate measurement of vitamin D in complex matrices such as foods and supplements has proved to present multiple challenges. Although isotope labeled vitamin D compounds are available as internal standard (IS) and useful to correct matrix effects, a possible significant error from the IS was reported by our group. It was found that the analyte and the IS may have different behaviors in the conversion between vitamin D and its pre-vitamin D isomer under specific conditions. In this study, further investigation was done to include analyte stability, which has shown to be an additive variable under different temperatures and reaction times. Our results suggested that both the analyte and the IS degraded, but more importantly, the degradation rates were different between these two compounds. This difference yielded additional uncertainty to the accuracy of measurement. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.covance.com/content/dam/covance/assetLibrary/posters/mh-asms11spdf.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
