NDLI: SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3

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SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3

Content Provider	MDPI
Author	Zhao, Dan Kang, Wen Wang, Yiwen Ge, Jiuyu Huang, Jianfeng Yang, Jie Yang, Weidong Tang, Xuna Xie, Sijing
Copyright Year	2021
Description	Human dental pulp stem cells (hDPSCs), which are characterized by self-renewal capacity and the ability of multilineage differentiation, have gained increased attention in regenerative medicine recently. Histone acetylation modulator proteins (HAMPs) are a protein family that mediates the modification and identification of histone acetylation and participates in various critical cellular processes. Here, we comprehensively surveyed the expression profile of HAMPs during osteoblast differentiation of hDPSCs and found that the HDAC class III pathway was upregulated, whereas the signal transducer and activator of transcription 3 (STAT3) signaling was downregulated during osteogenesis. Further laboratory research demonstrated that Sirtuin-1 (SIRT1), a class III HDAC, was upregulated and STAT3 activation was downregulated during osteogenic differentiation. SIRT1 counteracted the activation of STAT3 to promote osteogenic differentiation of hDPSCs at 7 and 21 days in both Western blot assay and chemical staining, which highlights the promising utility of SIRT1 activators in hDPSCs-based therapies for bone augmentation strategies and provides clinical insights that may lead to the development of osteogenic agents.
Starting Page	1353
e-ISSN	20796412
DOI	10.3390/coatings11111353
Journal	Coatings
Issue Number	11
Volume Number	11
Language	English
Publisher	MDPI
Publisher Date	2021-11-03
Access Restriction	Open
Subject Keyword	Coatings Dentistry and Oral Surgery Dental Pulp Stem Cells Sirt1 Stat3 Histone Acetylation Modulator Proteins Osteogenic Differentiation
Content Type	Text
Resource Type	Article

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