NDLI: Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway

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Hyaluronan-Based Gel Promotes Human Dental Pulp Stem Cells Bone Differentiation by Activating YAP/TAZ Pathway

Content Provider	MDPI
Author	Noce, Marcella La Stellavato, Antonietta Vassallo, Valentina Cammarota, Marcella Laino, Luigi Desiderio, Vincenzo Vecchio, Vitale Del Nicoletti, Giovanni Francesco Tirino, Virginia Papaccio, Gianpaolo Schiraldi, Chiara Ferraro, Giuseppe Andrea
Copyright Year	2021
Description	Background: Hyaluronans exist in different forms, accordingly with molecular weight and degree of crosslinking. Here, we tested the capability to induce osteogenic differentiation in hDPSCs (human dental pulp stem cells) of three hyaluronans forms: linear pharmaceutical-grade hyaluronans at high and (HHA) low molecular weight (LHA) and hybrid cooperative complexes (HCC), containing both sizes. Methods: hDPSCs were treated with HHA, LHA, HCC for 7, 14 and 21 days. The effects of hyaluronans on osteogenic differentiation were evaluated by qRT-PCR and WB of osteogenic markers and by Alizarin Red S staining. To identify the involved pathway, CD44 was analyzed by immunofluorescence, and YAP/TAZ expression was measured by qRT-PCR. Moreover, YAP/TAZ inhibitor-1 was used, and the loss of function of YAP/TAZ was evaluated by qRT-PCR, WB and immunofluorescence. Results: We showed that all hyaluronans improves osteogenesis. Among these, HCC is the main inducer of osteogenesis, along with overexpression of bone related markers and upregulating CD44. We also found that this biological process is subordinate to the activation of YAP/TAZ pathway. Conclusions: We found that HA’s molecular weight can have a relevant impact on HA performance for bone regeneration, and we unveil a new molecular mechanism by which HA acts on stem cells.
Starting Page	2899
e-ISSN	20734409
DOI	10.3390/cells10112899
Journal	Cells
Issue Number	11
Volume Number	10
Language	English
Publisher	MDPI
Publisher Date	2021-10-26
Access Restriction	Open
Subject Keyword	Cells Dentistry and Oral Surgery Hyaluronic Acid Dental Pulp Stem Cells Osteogenic Differentiation Yap/taz Pathway
Content Type	Text
Resource Type	Article

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