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| Content Provider | MDPI |
|---|---|
| Author | Hashim, Hairulazwan Maruyama, Hisataka Masuda, Taisuke Arai, Fumihito |
| Abstract | Manipulation and injection of single nanosensors with high cell viability is an emerging field in cell analysis. We propose a new method using fluorescence nanosensors with a glass nanoprobe and optical control of the zeta potential. The nanosensor is fabricated by encapsulating a fluorescence polystyrene nanobead into a lipid layer with 1,3,3-trimethylindolino-6′-nitrobenzopyrylospiran (SP), which is a photochromic material. The nanobead contains iron oxide nanoparticles and a temperature-sensitive fluorescent dye, Rhodamine B. The zeta potential of the nanosensor switches between negative and positive by photo-isomerization of SP with ultraviolet irradiation. The positively-charged nanosensor easily adheres to a negatively-charged glass nanoprobe, is transported to a target cell, and then adheres to the negatively-charged cell membrane. The nanosensor is then injected into the cytoplasm by heating with a near-infrared (NIR) laser. As a demonstration, a single 750 nm nanosensor was picked-up using a glass nanoprobe with optical control of the zeta potential. Then, the nanosensor was transported and immobilized onto a target cell membrane. Finally, it was injected into the cytoplasm using a NIR laser. The success rates of pick-up and cell immobilization of the nanosensor were 75% and 64%, respectively. Cell injection and cell survival rates were 80% and 100%, respectively. |
| File Size | 6175744 |
| File Format | |
| e-ISSN | 14248220 |
| DOI | 10.3390/s16122041 |
| Journal | Sensors |
| Issue Number | 12 |
| Volume Number | 16 |
| Language | English |
| Publisher Date | 2016-12-01 |
| Access Restriction | Open |
| Subject Keyword | nanosensor fluorescence sensor photochromism zeta potential cell injection |
| Content Type | Text |
| Resource Type | Article |
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