NDLI: Direct Identification of Human Oxytocin Receptor-binding Domains Using a Photoactivatable Cyclic Peptide Antagonist COMPARISON WITH THE HUMAN V1a VASOPRESSIN RECEPTOR

Content Provider	Journal of Biological Chemistry (JBC)
Author	Breton, Christophe Chellil, Hichem Kabbaj-Benmansour, Majida Carnazzi, Eric Seyer, René Phalipou, Sylvie Morin, Denis Durroux, Thierry Zingg, Hans Barberis, Claude Mouillac, Bernard
Abstract	Understanding of the molecular determinants responsible for antagonist binding to the oxytocin receptor should provide important insights that facilitate rational design of potential therapeutic agents for the treatment of preterm labor. To study ligand/receptor interactions, we used a novel photosensitive radioiodinated antagonist of the human oxytocin receptor, d(CH2)5 [Tyr(Me)2,Thr4,Orn8,Phe(3125I,4N3)-NH2 9]vasotocin. This ligand had an equivalent high affinity for human oxytocin and V1a vasopressin receptors expressed in Chinese hamster ovary cells. Taking advantage of this dual specificity, we conducted photoaffinity labeling experiments on both receptors. Photolabeled oxytocin and V1a receptors appeared as a unique protein band at 70–75 kDa and two labeled protein bands at 85–90 and 46 kDa, respectively. To identify contact sites between the antagonist and the receptors, the labeled 70–75- and the 46-kDa proteins were cleaved with CNBr and digested with Lys-C and Arg-C endoproteinases. The fragmentation patterns allowed the identification of a covalently labeled region in the oxytocin receptor transmembrane domain III consisting of the residues Leu114-Val115-Lys116. Analysis of contact sites in the V1a receptor led to the identification of the homologous region consisting of the residues Val126-Val127-Lys128. Binding domains were confirmed by mutation of several CNBr cleavage sites in the oxytocin receptor and of one Lys-C cleavage site in the V1a receptor. The results are in agreement with previous experimental data and three-dimensional models of agonist and antagonist binding to members of the oxytocin/vasopressin receptor family.
Related Links	http://www.jbc.org/content/276/29/26931.abstract
Ending Page	26941
Starting Page	26931
Page Count	11
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	29
Volume Number	276
DOI	10.1074/jbc.M102073200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2001-07-20
Access Restriction	Open
Subject Keyword	Oxytocin (OT) OT receptor (OTR) Three-dimensional (3D) Transmembrane domain (TMD) Arginine-vasopressin (AVP) D(CH2)5[Tyr(Me)2,Thr4,Orn8,Tyr(3I)-NH29]vasotocin (I-OTA) D(CH2)5[Tyr(Me)2,Thr4,Orn8,Tyr(3125I)-NH29]vasotocin (125I-OTA) D(CH2)5[Tyr(Me)2,Thr4,Orn8,Phe(3I,4N3)-NH29]vasotocin (I-ZOTA) D(CH2)5[Tyr(Me)2,Thr4,Orn8,Phe(3125I,4N3)-NH29]vasotocin (125I-ZOTA) Chinese hamster ovary (CHO) Bovine serum albumin (BSA) Inositol phosphate (IP) Polyacrylamide gel electrophoresis (PAGE) High pressure liquid chromatography (HPLC) N-[2-hydroxy-1,1-bis- (hydroxymethyl)ethyl]glycine (Tricine) 3-azidophenylpropionyl1-d-Tyr(Me)2-Phe3-Gln4-Asn5-Arg6-Pro7-Arg8-Tyr9-NH2 (3N3Phpa-LVA) 4-hydroxyphenylacetyl1-d-Tyr(Me)2-Phe3-Gln4-Asn5-Arg6-Pro7-Arg8-NH2 (HO-LVA) MECHANISMS OF SIGNAL TRANSDUCTION 4-hydroxyphenylpropionyl1-d-Tyr(Me)2-Phe3-Gln4-Asn5-Arg6-Pro7-Lys(3-azidophenylpropionyl)8-NH2 ([Lys(3N3Phpa)8]HO-LVA)
Alternative Title	Direct Identification of Human Oxytocin Receptor-binding Domains Using a Photoactivatable Cyclic Peptide Antagonist
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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Direct Identification of Human Oxytocin Receptor-binding Domains Using a Photoactivatable Cyclic Peptide Antagonist COMPARISON WITH THE HUMAN V1a VASOPRESSIN RECEPTOR

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