NDLI: Ykt6 Forms a SNARE Complex with Syntaxin 5, GS28, and Bet1 and Participates in a Late Stage in Endoplasmic Reticulum-Golgi Transport

Content Provider	Journal of Biological Chemistry (JBC)
Author	Zhang, Tao Hong, Wanjin
Abstract	The yeast SNARE Ykt6p has been implicated in several trafficking steps, including vesicular transport from the endoplasmic reticulum (ER) to the Golgi, intra-Golgi transport, and homotypic vacuole fusion. The functional role of its mammalian homologue (Ykt6) has not been established. Using antibodies specific for mammalian Ykt6, it is revealed that it is found mainly in Golgi-enriched membranes. Three SNAREs, syntaxin 5, GS28, and Bet1, are specifically associated with Ykt6 as revealed by co-immunoprecipitation, suggesting that these four SNAREs form a SNARE complex. Double labeling of Ykt6 and the Golgi marker mannosidase II or the ER-Golgi recycling marker KDEL receptor suggests that Ykt6 is primarily associated with the Golgi apparatus. Unlike the KDEL receptor, Ykt6 does not cycle back to the peripheral ER exit sites. Antibodies against Ykt6 inhibit in vitro ER-Golgi transport of vesicular stomatitis virus envelope glycoprotein (VSVG) only when they are added before the EGTA-sensitive stage. ER-Golgi transport of VSVG in vitro is also inhibited by recombinant Ykt6. In the presence of antibodies against Ykt6, VSVG accumulates in peri-Golgi vesicular structures and is prevented from entering the mannosidase II compartment, suggesting that Ykt6 functions at a late stage in ER-Golgi transport. Golgi apparatus marked by mannosidase II is fragmented into vesicular structures in cells microinjected with Ykt6 antibodies. It is concluded that Ykt6 functions in a late step of ER-Golgi transport, and this role may be important for the integrity of the Golgi complex.
Related Links	http://www.jbc.org/content/276/29/27480.abstract
Ending Page	27487
Starting Page	27480
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	29
Volume Number	276
DOI	10.1074/jbc.M102786200
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2001-07-20
Access Restriction	Open
Subject Keyword	N-ethylmaleimide-sensitive factor (NSF) SolubleN-ethylmaleimide-sensitive factor attachment protein (SNAP) Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) Endoplasmic reticulum (ER) Polyacrylamide gel electrophoresis (PAGE) Vesicular stomatitis virus envelope glycoprotein (VSVG) Vesicular stomatitis virus (VSV) Vesicular-tubular cluster (VTC) Intermediate compartment (IC) Fluorescein isothiocyanate (FITC) Glutathione S-transferase (GST) Guanosine 5′-3-O-(thio)triphosphate (GTPγS) Endoplasmic reticulum exit site (ERES) Endoplasmic reticulum-Golgi intermediate compartment (ERGIC) Phosphate-buffered saline (PBS) Endoglycosidase H (endo H) MEMBRANE TRANSPORT STRUCTURE FUNCTION AND BIOGENESIS
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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