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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Dembowski, Markus Künkele, Klaus-Peter Nargang, Frank E. Neupert, Walter Rapaport, Doron |
| Abstract | Translocation of preproteins across the mitochondrial outer membrane is mediated by the translocase of the outer mitochondrial membrane (TOM) complex. We report the molecular identification of Tom6 and Tom7, two small subunits of the TOM core complex in the fungus Neurospora crassa. Cross-linking experiments showed that both proteins were found to be in direct contact with the major component of the pore, Tom40. In addition, Tom6 was observed to interact with Tom22 in a manner that depends on the presence of preproteins in transit. Precursors of both proteins are able to insert into the outer membrane in vitro and are assembled into authentic TOM complexes. The insertion pathway of these proteins shares a common binding site with the general import pathway as the assembly of both Tom6 and Tom7 was competed by a matrix-destined precursor protein. This assembly was dependent on the integrity of receptor components of the TOM machinery and is highly specific as in vitro-synthesized yeast Tom6 was not assembled into N. crassa TOM complex. The targeting and assembly information within the Tom6 sequence was found to be located in the transmembrane segment and a flanking segment toward the N-terminal, cytosolic side. A hybrid protein composed of the C-terminal domain of yeast Tom6 and the cytosolic domain of N. crassaTom6 was targeted to the mitochondria but was not taken up into TOM complexes. Thus, both segments are required for assembly into the TOM complex. A model for the topogenesis of the small Tom subunits is discussed. |
| Related Links | http://www.jbc.org/content/276/21/17679.abstract |
| Ending Page | 17685 |
| Starting Page | 17679 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 21 |
| Volume Number | 276 |
| DOI | 10.1074/jbc.M009653200 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2001-05-25 |
| Access Restriction | Open |
| Subject Keyword | Translocase of the outer mitochondrial membrane (TOM) Translocase of the inner mitochondrial membrane (TIM) Blue native gel electrophoresis (BNGE) N-dodecyl β-d-maltoside (DDM) 1-ethyl-3-(3-dimethylaminopropyl)carbodiimidehydrochloride (EDC) Polymerase chain reaction (PCR) Outer membrane vesicles (OMV) Dihydrofolate reductase (DHFR) 4-morpholinepropanesulfonic acid (MOPS) Polyacrylamide gel electrophoresis (PAGE) 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (BisTris) MEMBRANE TRANSPORT STRUCTURE FUNCTION AND BIOGENESIS |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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