NDLI: The N-terminal Region of the Human Progesterone A-receptor STRUCTURAL ANALYSIS AND THE INFLUENCE OF THE DNA BINDING DOMAIN

Content Provider	Journal of Biological Chemistry (JBC)
Author	Bain, David L. Franden, Mary A. McManaman, James L. Takimoto, Glenn S. Horwitz, Kathryn B.
Abstract	The role of the N-terminal region in nuclear receptor function was addressed by a biochemical and biophysical analysis of the progesterone receptor A-isoform lacking only the hormone binding domain (NT-A). Sedimentation studies demonstrate that NT-A is quantitatively monomeric, with a highly asymmetric shape. Contrary to dogma, the N-terminal region is structured as demonstrated by limited proteolysis. However, N-terminal structure is strongly stabilized by the DNA binding domain, possibly explaining the lack of structure seen in isolated activation domains. Upon DNA binding, NT-A undergoes N-terminal mediated assembly, suggestive of DNA-induced allostery, and consistent with changes in protease accessibility of sites outside the DNA binding domain. Microsequencing reveals that protease-accessible regions are limited to previously identified phosphorylation motifs and to functional domain boundaries.
Related Links	http://www.jbc.org/content/275/10/7313.abstract
Ending Page	7320
Starting Page	7313
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	10
Volume Number	275
DOI	10.1074/jbc.275.10.7313
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2000-03-10
Access Restriction	Open
Subject Keyword	Progesterone receptor (PR) Hormone binding domain (HBD) Activation function (AF) B-upstream segment (BUS) Polyacrylamide gel electrophoresis (PAGE) Glutathione S-transferase (GST) Progesterone response element (PRE) Glucocorticoid receptor (GR) Dithiothreitol (DTT) DNA binding domain (DBD) N-terminal region of the A-receptor (NT-A) PROTEIN STRUCTURE AND FOLDING
Alternative Title	The N-terminal Region of the Human Progesterone A-receptor
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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