NDLI: Cloning of an Alkaline Ceramidase from Saccharomyces cerevisiae AN ENZYME WITH REVERSE (CoA-INDEPENDENT) CERAMIDE SYNTHASE ACTIVITY

Content Provider	Journal of Biological Chemistry (JBC)
Author	Mao, Cungui Xu, Ruijuan Bielawska, Alicja Obeid, Lina M.
Abstract	Ceramide is not only a core intermediate of sphingolipids but also an important modulator of many cellular events including apoptosis, cell cycle arrest, senescence, differentiation, and stress responses. Its turnover may be tightly regulated. However, little is known about the regulation of its metabolism because most enzymes responsible for its synthesis and breakdown have yet to be cloned. Here we report the cloning and characterization of the yeast gene YPC1 (YBR183w) by screeningSaccharomyces cerevisiae genes whose overexpression bestows resistance to fumonisin B1. We demonstrate that the yeast geneYPC1 encodes an alkaline ceramidase activity responsible for the breakdown of dihydroceramide and phytoceramide but not unsaturated ceramide. YPC1 ceramidase activity was confirmed byin vitro studies using an Escherichia coliexpression system. Importantly, YPC1p also has reverse activity, catalyzing synthesis of phytoceramide from palmitic acid and phytosphingosine. This ceramide synthase activity is CoA-independent and is resistant to fumonisin B1, thus explaining why YPC1was cloned as a fumonisin B1-resistant gene.
Related Links	http://www.jbc.org/content/275/10/6876.abstract
Ending Page	6884
Starting Page	6876
Page Count	9
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	10
Volume Number	275
DOI	10.1074/jbc.275.10.6876
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2000-03-10
Access Restriction	Open
Subject Keyword	TheYeast Phyto-ceramidase gene (YPC1) The product of the YPC1 gene (YPC1p) Geneticin (G418) Nucleotide (nt) DNA polymerase chain reaction (PCR) Open reading frame (ORF) Dihydrosphingosine (DHS) Dihydrosphingosine 1-phosphate (DHS-1-P) Phytosphingosine (PHS) Phytosphingosine 1-phosphate (PHS-1-P) Inositol phosphoceramide (IPC) Mannosylated IPC (MIPC) Endoplasmic reticulum (ER) LIPIDS AND LIPOPROTEINS
Alternative Title	Cloning of an Alkaline Ceramidase from Saccharomyces cerevisiae
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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