NDLI: A Role for Phospholipase D in GLUT4 Glucose Transporter Translocation

Content Provider	Journal of Biological Chemistry (JBC)
Author	Emoto, Masahiro Klarlund, Jes K. Waters, Steve B. Hu, Vivian Buxton, Joanne M. Chawla, Anil Czech, Michael P.
Abstract	Based on recent studies showing that phospholipase D (PLD)1 is associated with intracellular membranes and promotes membrane budding from the trans-Golgi, we tested its possible role in the membrane trafficking of GLUT4 glucose transporters. Using immunofluorescence confocal microscopy, expressed Myc epitope-tagged PLD1 was found to associate with intracellular vesicular structures by a mechanism that requires its N-terminal pleckstrin homology domain. Partial co-localization with expressed GLUT4 fused to green fluorescent protein in both 3T3-L1 adipocytes and Chinese hamster ovary cells was evident. Furthermore, microinjection of purified PLD into cultured adipocytes markedly potentiated the effect of a submaximal concentration of insulin to stimulate GLUT4 translocation to cell surface membranes. Insulin stimulated PLD activity in cells expressing high levels of insulin receptors but no such insulin effect was detected in 3T3-L1 adipocytes. Taken together, these results are consistent with the hypothesis that PLD1 associated with GLUT4-containing membranes acts in a constitutive manner to promote the mechanism of GLUT4 translocation by insulin.
Related Links	http://www.jbc.org/content/275/10/7144.abstract
Ending Page	7151
Starting Page	7144
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	10
Volume Number	275
DOI	10.1074/jbc.275.10.7144
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2000-03-10
Access Restriction	Open
Subject Keyword	Phosphatidylinositol (PI) Phorbol 12-myristate 13-acetate (PMA) Phospholipase D (PLD) ADP-ribosylation factor (ARF) Chinese hamster ovary cell (CHO) Phosphatidylinositol (PtdIns) Phosphatidylbutanol (PtdBuOH) Phosphatidylcholine (PtdCho) Hemagglutinin (HA) Pleckstrin homology (PH) Dulbecco's modified Eagle's medium (DMEM) Phosphate-buffered saline (PBS) Green fluorescent protein (GFP) MECHANISMS OF SIGNAL TRANSDUCTION
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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