NDLI: Studies on binding of single-stranded DNA with reduced graphene oxide

Content Provider	IET Digital Library
Author	Li, Xi Yang, Linqing Wang, Yunfei Du, Zhongyu Mao, Xuyan Sun, Dezhi Liu, Jun Zhou, Yu Xu, Xiangyu
Abstract	The binding reaction of reduced graphene oxide–silver nanocomposites (rGO–AgNCs) with calf thymus single-stranded DNA (ssDNA) was studied by ultraviolet–visible absorption, fluorescence spectroscopy and circular dichroism (CD), using berberine hemisulphate (BR) dye as a fluorescence probe. The absorbance of ssDNA increases, but the fluorescence intensity is quenched with the addition of rGO–AgNCs. The binding of rGO–AgNCs with ssDNA was able to increase the quenching effects of BR and ssDNA, and induce the changes in CD spectra. All of the evidence indicated that there was a relatively strong interaction between ssDNA and rGO–AgNCs. The data obtained from fluorescence experiments revealed that the quenching process of ssDNA caused by rGO–AgNCs is primarily due to complex formation, i.e. static quenching. The increasing trend of the binding equilibrium constant (Ka) with rising temperature indicated that the binding process was an endothermic reaction. The calculated thermodynamic parameters showed that the binding process was thermodynamically spontaneous, and hydrophobic association played predominant roles in the binding of ssDNA to the surface of rGO–AgNCs.
Starting Page	308
Ending Page	313
Page Count	6
ISSN	17518741
Volume Number	14
e-ISSN	1751875X
Issue Number	Issue 4, Jun (2020)
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/iet-nbt/14/4
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/10.1049/iet-nbt.2019.0377
Journal	IET Nanobiotechnology
Publisher Date	2020-02-25
Access Restriction	Open
Rights Holder	© The Institution of Engineering and Technology
Subject Keyword	Association Association, Addition, And Insertion Berberine Hemisulphate Dye Binding Equilibrium Constant Binding Process Binding Reaction Biochemistry Biomolecular Interactions Biomolecular Structure, Configuration, Conformation, And Active Sites Calculated Thermodynamic Parameter Calf Thymus Single-stranded DNA Charge Transfer Complexes Circular Dichroism CO-Ag Complex Formation DNA Dyes Electrochemistry And Electrophoresis Electronic Structure And Spectra of MacroMolecule Fluorescence Fluorescence Experiments Fluorescence Intensity Fluorescence Probe Fluorescence Spectroscopy Graphene Compound Hydrophobicity Interactions with Radiations at The Biomolecular Level Macromolecular Configuration (bonds, Dimensions) Molecular Biophysics Molecular Fluorescence And Phosphorescence Spectra Nanobiotechnology Nanocomposite Nanofabrication Optical Activity, Optical Rotation, Circular Dichroism in Molecule Physical Chemistry of Biomolecular Solutions And Condensed State Radiation Quenching Reduced Graphene Oxide-silver Nanocomposite RGO-AgNCs Binding Silver Compound Sorption And Accommodation Coefficient SsDNA Increases Static Quenching Surface Chemistry Ultraviolet Molecular Spectra Ultraviolet Spectra Ultraviolet-visible Absorption Visible Molecular Spectra Visible Spectra
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Biotechnology

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