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| Content Provider | IET Digital Library |
|---|---|
| Author | Xu, Peifeng Wu, Gangshan Xu, Chao Wang, Pan Yang, Ning Zhang, Rongbiao |
| Abstract | In view of the defects such as long time-consuming and large limitation in the detection of pesticide resistance of crop pathogens at present. A novel photoelectric detection method based on enriched microfluidic chips is proposed for the detection of pesticide resistance of crop fungal disease. First, according to the aerodynamics characteristics of microbial aerosol particles and optical detection technology, a special enrichment microfluidic chip was designed to achieve high efficiency enrichment and high precision concentration detection of fungal spores. On this basis, based on the theory of pesticide resistance sensitivity baseline, the relationship among spore germination, systematic enrichment concentration, and optical detection concentration of Botrytis cinerea in strawberry treated with Pyrimethanil was studied, and the pesticide resistance detection model of uncultured pathogens was established. The light intensity of light source was 1.1 × 103 cd, and the wavelength was 620 nm as the optimum parameters for optical detection. The correlation coefficient between the microscopic examination of spores’ concentration and the results of optical detection is 0.9792. It has good linearity and reliability, which provides a theoretical basis for the research and development of portable equipment for detecting pesticide resistance of crop diseases. |
| Starting Page | 8566 |
| Ending Page | 8571 |
| Page Count | 6 |
| Volume Number | 2019 |
| e-ISSN | 20513305 |
| Issue Number | Issue 23, Dec (2019) |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/joe/2019/23 |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/10.1049/joe.2018.9056 |
| Journal | The Journal of Engineering |
| Publisher | The Institution of Engineering and Technology |
| Publisher Date | 2019-03-25 |
| Access Restriction | Open |
| Rights License | Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) |
| Subject Keyword | Aerodynamics Aerosols Agriculture Agrochemicals Applied Fluid Mechanics Biological Technique BioMEMS Crop Diseases Crop Fungal Disease Crop Fungi Resistance Crop Pathogens Crops Engineering Material Enriched Microfluidic Chips Fluid Mechanics Food Product Food Safety Fungal Spores Health And Safety Aspects High Efficiency Enrichment High Precision Concentration Detection Industrial Processes Mechanical Engineering Microbial Aerosol Particle Microfluidic Chip Microfluidics Micromechanical And Nanomechanical Device And System Micromechanics Microorganisms Optical Detection Concentration Optical Detection Technology Pest Control Pesticide Resistance Detection Model Pesticide Resistance Sensitivity Baseline Photoelectric Detection Method Plant Diseases Product And Commodities Systematic Enrichment Concentration Wavelength 620.0 Nm |
| Content Type | Text |
| Resource Type | Article |
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