NDLI: Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells

Content Provider	IEEE Xplore Digital Library
Author	Yuchun Chen Li, P.C.H.
Copyright Year	2008
Description	Author affiliation: Dept. of Chem., Simon Fraser Univ., Burnaby, BC (Yuchun Chen; Li, P.C.H.)
Abstract	A single cancer cell was isolated and captured by a microfluidic single-cell biochip. The chip was made of glass and was fabricated by standard photolithography and wet etch process. Simultaneous optical observation and fluorescent measurement were achieved on the captured cancer cell. This was achieved by using red light to observe the cell; whereas the green fluorescent emission was measured photometrically to monitor the drug concentration. A CCD camera was used to image the cell morphology. At the same time, a PMT detector was used to monitor the cellular drug concentrations change in the single cell as a decrease in the cellular fluorescence intensity. Kinetic information of drug concentration change was obtained by analyzing the real time PMT data. This experiment revealed that during the efflux process, the anti-cancer drug (daunorubicin, DNR) decreased as the drug was pumped out of the cell because of multidrug resistance. The reversal effect of a multidrug resistance inhibitor (Sodium orthovanadate, OVN) on the cellular drug retention in single cancer cells was examined in this study. It was found that a higher cellular DNR concentration was resulted when OVN was present.
Starting Page	1
Ending Page	5
File Size	354563
Page Count	5
File Format	PDF
ISBN	9781424423958
DOI	10.1109/IMS3TW.2008.4581629
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-06-18
Publisher Place	Canada
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Drugs Semiconductor device measurement Fluorescence Optical variables measurement Reservoirs Microfluidics Cancer
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Fluorescence sensor array for non-contact measurement of oxygen consumption rate of single oocyte on a microfluidic chip

Microfluidic pressure measurements with optical trapping

Non-contact measurement of oxygen consumption rate of single oocyte using stripe-shaped fluorescence sensor on microfluidic chip

A novel microfluidic system for fluorescent sample analysis fabricated by rapid prototyping

Nanosecond fluorescence lifetime low-cost sensor

Lab on a chip fluorescence measurements — From single biomolecules to testing standards

Cell cytotoxicity analysis of adherent and suspended cancer cells using an integrated microfluidic device

Drug Accumulation Into Single Drug-Sensitive and Drug-Resistant Prostate Cancer Cells Conducted on the Single Cell Bioanalyzer

Imaging based optofluidic interferometer on a chip

Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells

Similar Documents

Fluorescence sensor array for non-contact measurement of oxygen consumption rate of single oocyte on a microfluidic chip

Microfluidic pressure measurements with optical trapping

Non-contact measurement of oxygen consumption rate of single oocyte using stripe-shaped fluorescence sensor on microfluidic chip

A novel microfluidic system for fluorescent sample analysis fabricated by rapid prototyping

Nanosecond fluorescence lifetime low-cost sensor

Lab on a chip fluorescence measurements — From single biomolecules to testing standards

Cell cytotoxicity analysis of adherent and suspended cancer cells using an integrated microfluidic device

Drug Accumulation Into Single Drug-Sensitive and Drug-Resistant Prostate Cancer Cells Conducted on the Single Cell Bioanalyzer

Imaging based optofluidic interferometer on a chip

Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells