NDLI: Cell cytotoxicity analysis of adherent and suspended cancer cells using an integrated microfluidic device

Content Provider	IEEE Xplore Digital Library
Author	Huixue Song Shaotie Peng Tan Chen Baoyue Zhang Zhanhui Wang
Copyright Year	2011
Description	Author affiliation: Key Lab of Health Informatics of Chinese Academy of Sciences, Institute of Biomedical and Health Engineering, Shenzhen, Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, P.R. China 518055 (Huixue Song; Shaotie Peng; Tan Chen; Baoyue Zhang; Zhanhui Wang)
Abstract	In this paper, a multilayer elastomeric microfluidic device with micro cell sieves was developed for an assay of drug induced cytotoxicity. We evaluated the cytotoxic effects of anti-cancer drugs tamoxifen (TAM) and camptothecin using MDA-MB-231 breast cancer cells and human chronic myeloid leukaemia K562 cells respectively. To validate cell morphology and viability on the microfluidic chip, fluorescent cell viability assay was carried out using three fluorescent dyes. The results showed that TAM and camptothecin both induced cytotoxicity of the two cell types in a concentration-dependent manner. Using this scalable platform, cell seeding and anti-cancer drugs exposure can be carried out within a single microfluidic device in a multiplexed format, enabling high-density parallel cytotoxicity screening while minimizing reagent consumption.
Starting Page	1051
Ending Page	1054
File Size	1182855
Page Count	4
File Format	PDF
ISBN	9781424493517
e-ISBN	9781424493524
DOI	10.1109/BMEI.2011.6098477
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-10-15
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Drugs microfluidic device K562 cells Materials Fluorescence Valves MDA-MB-231 cells anti-cancer drugs Arrays Microfluidics cytotoxicity Cancer
Content Type	Text
Resource Type	Article

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