NDLI: Calcium imaging in temporal focus

Content Provider	IEEE Xplore Digital Library
Author	Oshiorenoya, A.E. Marchand, P. Mutlu, M. Klotz, L. Schultz, S.R.
Copyright Year	2013
Description	Author affiliation: Cellular Imaging & Electrophysiology Lab., Bogazici Univ., Istanbul, Turkey (Mutlu, M.; Schultz, S.R.) \|\| Dept. of Bioeng., Imperial Coll. London, London, UK (Oshiorenoya, A.E.; Marchand, P.; Klotz, L.)
Abstract	Techniques for fast two photon laser scanning microscopy (2PLSM) of dynamic neural signals offer the prospect of minimally invasive functional mapping of neural circuitry. In a typical imaging scenario, a two-photon point spread function is raster scanned across a region of tissue, and pixels within regions of interest (e.g neuronal somata) are averaged to form a point in a time series. In galvanometric microscopes, this means that scan time is wasted in moving the point spread function around the cell, limiting signal acquisition rate. As a means of addressing this issue, we propose the use of temporally focused (TEFO) 2PLSM for monitoring dynamic signals. In contrast to the diffraction limited spot of 2PLSM, the TEFO spot is several orders of magnitude larger. It nonetheless preserves the axial confinement of the 2PLSM spot and also propagates such that multiphoton absorption is confined to the perifocal region. We demonstrate proof of principal by employing TEFO 2PLSM to image calcium signals in a thalamocortical slice stained with the calcium sensitive AM-ester dye (OGB-1 AM). We acquired $Ca^{2+}$ signals in 2D line-scanning mode, at scan rates of 1 kHz, showing that action potential detection with relatively high temporal resolution can be achieved with this approach.
Starting Page	1525
Ending Page	1528
File Size	1910268
Page Count	4
File Format	PDF
ISBN	9781467319690
ISSN	19483554
DOI	10.1109/NER.2013.6696236
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-11-06
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Integrated optics Biomedical optical imaging Microscopy Calcium Optical imaging Photonics
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Hollow-core photonics for optofluidics and atom photonics

Wafer-Fusion-Enabled Platforms for Mid-Infrared Integrated Silicon Photonics

Nanowire Photonics

Silicon photonics for on-chip spectrophotometry

Current trend and recent progresses in optical trapping: from optical tweezers to single-particle spectroscopy and photonics force microscopy

Scanning probe optical microscopy using an integrated submicron organic photodetector

Optical Characterization of Electronic Packages with Confocal Microscopy

Rethinking microscopy

New advances of nonlinear optical microscopy

Calcium imaging in temporal focus

Similar Documents

Hollow-core photonics for optofluidics and atom photonics

Wafer-Fusion-Enabled Platforms for Mid-Infrared Integrated Silicon Photonics

Nanowire Photonics

Silicon photonics for on-chip spectrophotometry

Current trend and recent progresses in optical trapping: from optical tweezers to single-particle spectroscopy and photonics force microscopy

Scanning probe optical microscopy using an integrated submicron organic photodetector

Optical Characterization of Electronic Packages with Confocal Microscopy

Rethinking microscopy

New advances of nonlinear optical microscopy

Calcium imaging in temporal focus