NDLI: Micropatterned polymer substrates control alignment of proliferating Schwann cells to direct neuronal regeneration

Content Provider	IEEE Xplore Digital Library
Author	Schmalenberg, K.E. Uhrich, K.E.
Copyright Year	2003
Description	Author affiliation: Dept. of Chem. & Chem. Biol., Rutgers Univ., Piscataway, NJ, USA (Schmalenberg, K.E.; Uhrich, K.E.)
Abstract	In this study we have examined the use of microcontact printed polymeric substrates for their ability to control Schwann cell attachment and direct proliferation. Schwann cell guidance is a crucial factor in directing peripheral nerve regeneration. Elastomeric stamps of poly(dimethyl siloxane) were created from lithographic masters. The stamp pattern consisted of stripes and spaces 10-50 /spl mu/m wide. The substrates including poly(methyl methacrylate), poly(hydroxybutyrate), and poly(/spl epsi/-caprolactone) were plasma treated along with the stamps. The stamps were "inked" with laminin, a permissive protein for Schwann cell adhesion, and stamped onto the substrates. Schwann cells were seeded onto the substrates in serum free media. After 24 hrs media was replaced with serum-containing growth media and changed daily thereafter. Phase contrast images were taken at 24 hr intervals after seeding. After monolayer formation, the samples were fixed and fluorescently labeled. We found that the laminin micropatterns directed Schwann cell adhesion to the patterned areas when seeded in serum free media. The addition of a growth media to stimulate proliferation caused some loss in orientation control initially, but when monolayer formation was complete a high degree of orientation could be observed. Our results indicate that patterned polymeric substrates may enhance peripheral nerve regeneration by creating a highly ordered Schwann cell matrix for guidance of neurons.
Starting Page	245
Ending Page	248
File Size	703795
Page Count	4
File Format	PDF
ISBN	0780375793
DOI	10.1109/CNE.2003.1196804
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2003-03-20
Publisher Place	Italy
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Chemistry Adhesives Neurons Cells (biology) Plasmas Polymers Surface treatment Chemicals Biological materials
Content Type	Text
Resource Type	Article

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