NDLI: Development and preliminary application of TaqMan fluorescence quantitative RT-PCR assay for detection of porcine Sapovirus

Content Provider	IEEE Xplore Digital Library
Author	Zhichao Wang Xiuguo Hua Yan Chen Li Cui Peng Wang Weiqi Li
Copyright Year	2010
Description	Author affiliation: Shanghai Realgene Technology Inc, China (Peng Wang; Weiqi Li) \|\| Shanghai Key Laboratory of Veterinary Biotechnology, Shanghai Jiaotong University, China (Zhichao Wang; Xiuguo Hua; Yan Chen; Li Cui)
Abstract	The primers and probes were designed and synthesized according to the conserved VP1 sequences of porcine Sapovirus (SaV), and a TaqMan fluorescence quantitative RT-PCR assay were developed by optimizing the reaction conditions. Results showed that the fluorescence quantitative RT-PCR assay could detect 16.1 $copies·µL^{−1}$ of plasmid DNA, while the sensitivity of the routine RT-PCR was 1.61 × $10^{3}$ $copies·µL^{−1}.$ 216 stool samples were then detected by the established quantitative RT-PCR assay, and the results were compared with that of routine RT-PCR. It also showed that the sensitivity of established method was higher than that of the routine RT-PCR. Phylogenetic analysis indicated that all of the 4 SaV strains we had identified belonged to G?, and shared 100% nucleotide homology with another Shanghai porcine SaV strain (FJ387164). The TaqMan fluorescence quantitative PCR assay, which is more specific, sensitive and accurate, can be used for the epidemiological investigation and diagnosis of porcine SaV infection.
Starting Page	2012
Ending Page	2016
File Size	397058
Page Count	5
File Format	PDF
ISBN	9781424464951
e-ISBN	9781424464982
DOI	10.1109/BMEI.2010.5639465
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-10-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Real time systems Sensitivity RNA fluorescence quantitative RT-PCR Humans Fluorescence TaqMan probe porcine Sapovirus Probes Strain
Content Type	Text
Resource Type	Article

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