NDLI: New technologies for nanobiosensing and their applications to real-time monitoring

Content Provider	IEEE Xplore Digital Library
Author	Boero, C. Carrara, S. De Micheli, G.
Copyright Year	2011
Description	Author affiliation: EPFL - École Politechnique Fédérale de Lausanne, 1015 - Switzerland (Boero, C.; Carrara, S.; De Micheli, G.)
Abstract	Amperometric biosensors are complex systems and they require a combination of technologies for their development. The aim of the present work is to exploit existing technologies for new purposes in order to develop nanostructured biosensors for the real-time detection of multiple metabolites in cell culture flasks. The fabrication of five Au working electrodes onto silicon substrate is achieved with CMOS compatible microtechnology. Each working electrode presents an area of 0.25 $mm^{2},$ so structuration with carbon nanotubes and specific functionalization are carried out by using spotting technology, originally developed for microarrays and DNA printing. The electrodes are characterized by cyclic voltammetry and compared with commercially available screen-printed electrodes. Finally, they are functionalized with different enzymes and calibrated for real-time detection of glutamate, glucose and lactate under flow conditions. The developed biosensor for real-time and online detection of multiple metabolites shows very promising results towards its application in cell culture monitoring.
Starting Page	357
Ending Page	360
File Size	918559
Page Count	4
File Format	PDF
ISBN	9781457714696
e-ISBN	9781457714702
DOI	10.1109/BioCAS.2011.6107801
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-11-10
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrodes Real time systems Carbon nanotubes Calibration Biosensors Sugar Monitoring
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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