NDLI: Enhancement of ultrasonically induced cell transfection with artificial cavitation nuclei

Content Provider	IEEE Xplore Digital Library
Author	Greenleaf, W.J. Bolander, M.E. Sarkar, G. Goldring, M.B. Greenleaf, J.F.
Copyright Year	1997
Description	Author affiliation: Mayo High Sch., Rochester, MN, USA (Greenleaf, W.J.)
Abstract	Ultrasound mediated gene transfection in cultured immortalized human chondrocytes was enhanced three-fold using artificial cavitation nuclei in the form of Albunex(R). 1.0 MHz ultrasound transmitted through the bottoms of six-well culture plates containing media with green fluorescent protein reporter gene plasmids at a concentration of 40 /spl mu/g/ml and Albunex(R) at 50/spl times/10/sup 6/ bubbles/ml, exhibited a peak transfection efficiency of about 50% of the living cells when exposed to 4/spl times/10/sup 5/ Pascals spatial average peak pressure (SAPP) for 20 minutes. Using these parameters, transfection efficiency increased linearly with ultrasound exposure pressure with a transfection threshold observed at a SAPP of 10/sup 5/ Pascals. Adding fresh Albunex(R) at 50/spl times/10/sup 6/ bubbles/ml prior to sequential one second 3.2 or 4.0/spl times/10/sup 5/ Pascal exposures increased transfection efficiency by 15% for each of three consecutive exposures. Efficient in vitro and perhaps in vivo transfection appears possible with ultrasound.
Starting Page	1365
Ending Page	1369
File Size	357098
Page Count	5
File Format	PDF
ISBN	0780341538
ISSN	10510117
DOI	10.1109/ULTSYM.1997.661830
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	1997-10-05
Publisher Place	Canada
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Ultrasonic imaging Proteins DNA In vivo Humans Gene therapy Acoustic testing Protocols USA Councils Orthopedic surgery
Content Type	Text
Resource Type	Article

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