NDLI: A numerical optimization approach for color correction in forensic DNA genotyping

Content Provider	IEEE Xplore Digital Library
Author	El-Difrawy, S. Ehrlich, D.
Copyright Year	2003
Description	Author affiliation: Whitehead Inst. for Biomed. Res., Cambridge, MA, USA (El-Difrawy, S.; Ehrlich, D.)
Abstract	Recent advances in sequencing technology made short tandem repeats (STR) genotyping a leading tool in forensic DNA analysis. STR genotyping is performed using electrophoresis where DNA strands with different fluorescence labeling dyes are separated according to their length. Shorter strands arrive earlier at the detector where the attached dyes are excited by an induced laser. The resulting excitation is collected using a multichannel detector, with each channel tuned to detect the strength of a certain labeling dye, utilizing photomultiplier tubes or CCD devices. The spectrum overlap of the dyes causes a detector tuned to the wavelength of a specific dye to also detect with different sensitivity other dyes. In this work we propose a new numerical based algorithm for correcting cross talk resulting from such spectrum overlap. We discuss reasons earlier solutions to a similar problem can not be applied in the genotyping setting. We test our proposed algorithm and report preliminary results showing the success of the algorithm.
Starting Page	2088
Ending Page	2092
File Size	342984
Page Count	5
File Format	PDF
ISBN	0780381041
DOI	10.1109/ACSSC.2003.1292348
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2003-11-09
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Color Forensics DNA Labeling Laser excitation Detectors Electrokinetics Fluorescence Laser tuning Photomultipliers
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

High-performance forensic DNA profiling using fluorescence energy transfer primers and a 96-lane microfabricated capillary array electrophoresis device

FRET imaging by picosecond TCSPC laser scanning microscopy

High sensitivity radiation detector for capillary electrophoresis

Separation imaging of DNA fragments in poly (dimethylsiloxane) microchip

Capillary zone electrophoresis in the forensic analysis of explosives

UV Laser Detection In Capillary Electrophoresis

Visualization of Joule Heating in Micro-Electrophoretic Flow Using 2-Color Laser-Induced Fluorescence

Laser diagnostics of a one-dimensional ordered ion beam

Vacuum-field level shifts in a single atom mediated by a single distant mirror

A numerical optimization approach for color correction in forensic DNA genotyping

Similar Documents

High-performance forensic DNA profiling using fluorescence energy transfer primers and a 96-lane microfabricated capillary array electrophoresis device

FRET imaging by picosecond TCSPC laser scanning microscopy

High sensitivity radiation detector for capillary electrophoresis

Separation imaging of DNA fragments in poly (dimethylsiloxane) microchip

Capillary zone electrophoresis in the forensic analysis of explosives

UV Laser Detection In Capillary Electrophoresis

Visualization of Joule Heating in Micro-Electrophoretic Flow Using 2-Color Laser-Induced Fluorescence

Laser diagnostics of a one-dimensional ordered ion beam

Vacuum-field level shifts in a single atom mediated by a single distant mirror

A numerical optimization approach for color correction in forensic DNA genotyping