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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Iordanov, V.P. Iliev, B.P. Joseph, V. Bossche, A. Bastemeijer, J. Sarro, P.M. Young, I.T. van Dedem, G.W.K. Vellekoop, M.J. |
| Copyright Year | 2004 |
| Description | Author affiliation: DIMES, Delft Univ. of Technol., Netherlands (Iordanov, V.P.; Iliev, B.P.) |
| Abstract | This paper presents thermal analysis verification of a sensorized 50 nl reactor chamber for DNA amplification based on PCR (polymerase chain reaction). The reactor is equipped with an integrated heater, temperature sensor and a photo detector for real time detection. Through micromachining, the thermal capacity of each chamber is minimized, enabling rapid PCR cycling. The proposed structure was implemented on a silicon substrate using a standard CMOS process and postprocessing. The chambers have a bottom area of 500/spl times/500 /spl mu/m/sup 2/ and a pitch of 1 mm. An array of 96 reactors can be formed on a square centimeter. In order to reach the required PCR temperature levels (55/spl deg/C, 75/spl deg/C and 92/spl deg/C) dedicated electronics, based on a proportional-integral (PI) controller were designed and built. The system is capable of stabilizing the temperature of the reactor and performing a temperature sweep up to 100/spl deg/C. |
| Starting Page | 229 |
| Ending Page | 232 |
| File Size | 403465 |
| Page Count | 4 |
| File Format | |
| ISBN | 0780386922 |
| DOI | 10.1109/ICSENS.2004.1426143 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2004-10-24 |
| Publisher Place | Austria |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Inductors DNA Polymers Temperature sensors Detectors Micromachining Rapid thermal processing Silicon CMOS process Pi control |
| Content Type | Text |
| Resource Type | Article |
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