Please wait, while we are loading the content...
| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Perni, S. Shama, G. Chalise, P.R. Kong, M. |
| Copyright Year | 2006 |
| Description | Author affiliation: Dept. of Chem. Eng., Loughborough Univ. (Perni, S.; Shama, G.) |
| Abstract | Summary form only given. Pulsed electric fields (PEF) of nanosecond duration offer an interesting new approach to the bacterial decontamination of liquids. We have designed, fabricated and tested a nanosecond PEF device in our laboratory. The device was operated at a constant electrical field intensity of 100 kV/cm with pulse durations of 32 ns and a repetition rate of 30 pulses per second. E. coli K12 cell suspensions in buffered solution in 0.1 ml cuvettes were exposed to nanosecond pulse trains of up to 5 minutes (9000 pulses). Bacterial inactivation was assessed by means of plating onto both ordinary tryptone soya agar (TSA), and onto TSA supplemented with either 2, 3 or 4% (w/v) NaCl. The latter imposes a stress on treated cells and only those cells that are wholly uninjured are able to grow at the highest concentration of NaCl used here. By conducting counts both 24 and 48 hrs after plating, we were also able to assess the length of time which sub-lethally injured cells required to recover from their injuries. We observed a distribution of both lethal and sub-lethal injury throughout the period of exposure to nanosecond PEF. Our results point to the accumulation of injurious events over time as the mechanism of cell death and suggest that once a threshold of damage is exceeded cells become non-viable. This would appear to be at odds with the previously widely held view that exposure of microbial cells to nanosecond pulses leads to irreversible membrane breakdown. These findings may have implications for the types of liquids that can successfully be treated by this technique |
| Sponsorship | Plasma Sci. and Applications Comm. IEEE Nuclear and Plasma Sci. Soc |
| Starting Page | 431 |
| Ending Page | 431 |
| File Size | 102197 |
| Page Count | 1 |
| File Format | |
| ISBN | 1424401259 |
| DOI | 10.1109/PLASMA.2006.1707304 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2006-06-04 |
| Publisher Place | USA |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Microorganisms Liquids Injuries Decontamination Testing Nanoscale devices Laboratories Suspensions Stress Lead |
| Content Type | Text |
| Resource Type | Article |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|