NDLI: On the alignment of transmission electron microscope images without fiducial markers

Content Provider	IEEE Xplore Digital Library
Author	Brandt, S. Heikkonen, J. Engelhardt, P.
Copyright Year	2002
Description	Author affiliation: Lab. of Computational Eng., Helsinki Univ. of Technol., Finland (Brandt, S.; Heikkonen, J.)
Abstract	Accurate image alignment is needed for computing three-dimensional reconstructions from transmission electron microscope tilt series. So far, the best alignment results have been obtained by using colloidal gold beads as fiducial markers. If their use has not been possible for some reason, the only option has been the automatic 2D cross-correlation-based registration methods. However, since the actual motion is three dimensional, this approach is inaccurate and inappropriate for the whole problem. Conversely, we propose a novel method that uses the actual 3D motion model without any fiducial markers in the images. The method is based on matching and tracking corner features by first solving the underlying geometrical constraint of consecutive images in the tilt series. The results show that our method is competitive with the gold marker alignment in the level of accuracy and hence opens the way for new opportunities in the analysis of electron tomography reconstructions.
Starting Page	278
Ending Page	281
File Size	321684
Page Count	4
File Format	PDF
ISBN	076951695X
ISSN	10514651
DOI	10.1109/ICPR.2002.1048294
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-08-11
Publisher Place	Canada
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Transmission electron microscopy Gold Tomography Image reconstruction Geometry Laboratories Image converters Head Maximum likelihood detection Maximum likelihood estimation
Content Type	Text
Resource Type	Article
Subject	Computer Vision and Pattern Recognition

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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