NDLI: Neurofilament tracking by detection in fluorescence microscopy images

Content Provider	IEEE Xplore Digital Library
Author	Junda Zhu Liang Yuan
Copyright Year	2013
Description	Author affiliation: Sch. of Mech. Eng., Xinjiang Univ., Urumqi, China (Liang Yuan) \|\| Fac. of Sci. & Technol., Univ. of Macau, Taipa, China (Junda Zhu)
Abstract	Neurofilaments are functional protein polymers that are transported along the axonal processes of nerve cells. Studying the movement of neurofilaments is important in applications such as diagnosing neurodegenerative diseases. Traditional methods have been largely relying on manual labeling of the neurofilaments in fluorescence microscopy images. In this paper, we describe an automated method for analyzing the motion of neurofilaments based on tracking-by-detection. The axon along which the neurofilaments move is extracted and represented by a parametric curve. The axon is decomposed into blocks, and the blocks encompassing the moving neurofilaments are determined by graph labeling using Markov Random Field. The leading and trailing locations of a neurofilament are then refined to sub-pixel accuracy. We demonstrate the efficacy and efficiency of our proposed method by experiments on real time-lapse fluorescence image sequences of neurofilament movement.
Starting Page	3123
Ending Page	3127
File Size	2284038
Page Count	5
File Format	PDF
ISBN	9781479923410
DOI	10.1109/ICIP.2013.6738643
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-09-15
Publisher Place	Australia
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	fluorescence microscopy Markov Random Field (MRF) graph cut
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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